Cooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stress.
Authors Wong Chi-Ming; Zhou Yuan; Ng Raymond W M; Kung Hf Hsiang-fu; Jin Dong-Yan;
JournalJ Biol Chem
PubMed ID11741925
'Peroxiredoxins are a family of antioxidant enzymes conserved from bacteria to humans. In Saccharomyces cerevisiae, there exist five peroxiredoxins, among which Tsa2p shares striking homology with the well described Tsa1p but has not been extensively studied. Here we report on the functional characterization of yeast tsa2Delta mutants and the comparison ... More
Structural consequences of cardiac troponin I phosphorylation.
AuthorsWard DG, Cornes MP, Trayer IP,
JournalJ Biol Chem
PubMed ID12207022
'beta-Adrenergic stimulation of the heart results in bisphosphorylation of the N-terminal extension of cardiac troponin I (TnI). Bisphosphorylation of TnI reduces the affinity of the regulatory site on troponin C (TnC) for Ca(2+) by increasing the rate of Ca(2+) dissociation. What remains unclear is how the phosphorylation signal is transmitted ... More
A CDC45 homolog in Arabidopsis is essential for meiosis, as shown by RNA interference-induced gene silencing.
'CDC45 is required for the initiation of DNA replication in yeast and cell proliferation in mammals and functions as a DNA polymerase alpha loading factor in Xenopus. We have cloned a CDC45 homolog from Arabidopsis whose expression is upregulated at the G1/S transition and in young meiotic flower buds. One-third ... More
Optimization of fusion PCR for in vitro construction of gene knockout fragments.
Authors Wang Hong-Liang; Postier Bradley L; Burnap Robert L;
JournalBiotechniques
PubMed ID12139251
This paper contains a comparison of proofreading polymerases including Platinum Pfx and Turbo Pfu.
mRNA PCR-based epitope chase method.
AuthorsDoucet JD, Gauchat D, Lapointe R
JournalMethods Mol Biol
PubMed ID23296942
The identification of specific viral and tumor antigen epitopes recognized by CD4(+) or CD8(+) T lymphocytes remains a challenge. Unfortunately, epitope mapping methods are generally costly and time-consuming. This chapter details a polymerase chain reaction (PCR)-based mRNA epitope identification method called mPEC, which is designed to rapidly and precisely identify ... More
Fetal fibronectin signaling induces matrix metalloproteases and cyclooxygenase-2 (COX-2) in amnion cells and preterm birth in mice.
AuthorsMogami H, Kishore AH, Shi H, Keller PW, Akgul Y, Word RA
JournalJ Biol Chem
PubMed ID23184961
Fetal fibronectin (fFN) in cervical and vaginal secretions has been used as a predictor of preterm delivery. Here, we clarified the pathological function of fFN on cell type-specific matrix metalloproteinases (MMPs) and prostaglandin synthesis in fetal membranes. Treatment of amnion mesenchymal cells with fFN resulted in dramatic increases in MMP-1 ... More