Identification of a Novel Transcription Factor, GAGATA-binding Protein, Involved in Androgen-mediated Expression of Prostate-specific Antigen.
AuthorsWang C, Yeung F, Liu PC, Attar RM, Geng J, Chung LW, Gottardis M, Kao C,
JournalJ Biol Chem
PubMed ID12782640
'Prostate-specific antigen (PSA) is the most valuable marker for the evaluation of prostate cancer progression. The expression of PSA is controlled by androgen receptor (AR) through its binding to androgen-response elements (AREs). Several AREs have been identified within the 5.8-kb PSA promoter. The main activity of this 5.8-kb PSA promoter ... More
A CDC45 homolog in Arabidopsis is essential for meiosis, as shown by RNA interference-induced gene silencing.
'CDC45 is required for the initiation of DNA replication in yeast and cell proliferation in mammals and functions as a DNA polymerase alpha loading factor in Xenopus. We have cloned a CDC45 homolog from Arabidopsis whose expression is upregulated at the G1/S transition and in young meiotic flower buds. One-third ... More
Optimization of fusion PCR for in vitro construction of gene knockout fragments.
Authors Wang Hong-Liang; Postier Bradley L; Burnap Robert L;
JournalBiotechniques
PubMed ID12139251
This paper contains a comparison of proofreading polymerases including Platinum Pfx and Turbo Pfu.
mRNA PCR-based epitope chase method.
AuthorsDoucet JD, Gauchat D, Lapointe R
JournalMethods Mol Biol
PubMed ID23296942
The identification of specific viral and tumor antigen epitopes recognized by CD4(+) or CD8(+) T lymphocytes remains a challenge. Unfortunately, epitope mapping methods are generally costly and time-consuming. This chapter details a polymerase chain reaction (PCR)-based mRNA epitope identification method called mPEC, which is designed to rapidly and precisely identify ... More
Fetal fibronectin signaling induces matrix metalloproteases and cyclooxygenase-2 (COX-2) in amnion cells and preterm birth in mice.
AuthorsMogami H, Kishore AH, Shi H, Keller PW, Akgul Y, Word RA
JournalJ Biol Chem
PubMed ID23184961
Fetal fibronectin (fFN) in cervical and vaginal secretions has been used as a predictor of preterm delivery. Here, we clarified the pathological function of fFN on cell type-specific matrix metalloproteinases (MMPs) and prostaglandin synthesis in fetal membranes. Treatment of amnion mesenchymal cells with fFN resulted in dramatic increases in MMP-1 ... More