Platinum™ Quantitative PCR SuperMix-UDG
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Citations & References (1)
Invitrogen™

Platinum™ Quantitative PCR SuperMix-UDG

**********************Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’veRead more
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Catalog NumberNo. of Reactions
11730025500 Reactions
11730017100 Reactions
Catalog number 11730025
Price (CNY)
11,581.00
Each
Add to cart
No. of Reactions:
500 Reactions
Price (CNY)
11,581.00
Each
Add to cart
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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.

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Platinum™ Quantitative PCR SuperMix-UDG is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2). The system is optimized to provide superior performance with either LUX™ Fluorogenic Primers or probe-based detection systems.

Platinum™ Quantitative PCR SuperMix-UDG:

• Is highly sensitive and specific
• Allows room temperature reaction assembly (3)
• Is easy to use-just add template and fluorogenic primers or probes for detection
• Includes a vial of 50 mM MgCl2 to accommodate a variety of primer/template PCR systems
• Is compatible with most real-time instruments

Applications: Real-time quantitative amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, and fluorometric SNP (Single Nucleotide Polymorphism) detection.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
No. of Reactions500 Reactions
Product LinePlatinum
Product TypeQuantitative PCR SuperMix-UDG
Quantity500 reactions
Sample TypeDNA (Genomic), cDNA
Shipping ConditionDry Ice
Sufficient For500 Reactions
Detection MethodPrimer-probe
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Platinum™ Quantitative PCR SuperMix-UDG is supplied in a 2X concentration [40 mM Tris-HCl (pH 8.4), 100 mM KCl, 6 mM MgCl2 , 400 μM dATP, 400 μM dCTP, 400 μM dGTP, 800 μM dUTP, 40 units/ml UDG, 60 units/ml Platinum™ Taq DNA Polymerase, stabilizers]. A vial of 50 mM MgCl2 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

I want to perform qRT-PCR using cDNA that I synthesize directly from cells. Can I use the SuperScript III CellsDirect cDNA Kit for end-point PCR and the Platinum Quantitative PCR SuperMix-UDG kit?

We would recommend using our Cells-to-CT kits for best qRT-PCR results. The CellsDirect kits for end-point PCR have not been optimized for qRT-PCR.

Why is UDG important in the Platinum Quantitative PCR SuperMix-UDG mix?

UDG and dUTP are included to prevent the reamplification of carryover PCR products between reactions. dUTP ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs.

Citations & References (1)

Citations & References
Abstract
Development and validation of a rotor-gene real-time PCR assay for detection, identification, and quantification of Chlamydia trachomatis in a single reaction.
Authors:Jalal H, Stephen H, Curran MD, Burton J, Bradley M, Carne C,
Journal:J Clin Microbiol
PubMed ID:16390971
A multitarget real-time PCR (MRT-PCR) for detection of Chlamydia trachomatis DNA was developed and validated. There were three targets for amplification in a single reaction: the cryptic plasmid (CP), the major outer membrane protein (MOMP) gene, and an internal control. The assay had the following characteristics: (i) detection and confirmation ... More