Analysis of one-step and two-step real-time RT-PCR using SuperScript III.
AuthorsWacker MJ,Godard MP
JournalJournal of biomolecular techniques : JBT
PubMed ID16461951
Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription carried out in the same tube as PCR) and two-step (reverse transcription carried out in a separate reaction) RT-PCR ... More
Design and validation of an H5 TaqMan real-time one-step RT-PCR and confirmatory assays for detection and verification of influenza A H5 infections in humans.
AuthorsEllis JS, Smith JD, Braham S, Lock M, Barlow K, Zambon MC,
JournalJ Clin Microbiol
PubMed ID17360836
Increasing diversity among influenza H5N1 viruses has resulted in the need for sensitive and specific diagnostic assays, fully validated for the detection of H5 viruses belonging to all HA clades, particularly recently circulating clade 2 H5N1 viruses. In this report the development and validation of a real-time one-step TaqMan RT-PCR ... More