SuperScript™ IV VILO™ 预混液
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
SuperScript™ IV VILO™ 预混液
Invitrogen™

SuperScript™ IV VILO™ 预混液

SuperScript™ IV VILO™ (SSIV VILO) 预混液是一种反应预混液,用于在RT-qPCR 中快速、灵敏且可重复地检测 cDNA 的合成了解更多信息
Have Questions?
更改视图buttonViewtableView
货号包括反应次数
11766500Kit with ezDnase500 次反应
11756050Kit only50 次反应
11756500Kit only500 次反应
11766050Kit with ezDnase50 次反应
货号 11766500
价格(CNY)
70,116.00
Each
添加至购物车
包括:
Kit with ezDnase
反应次数:
500 次反应
请求批量或定制报价
价格(CNY)
70,116.00
Each
添加至购物车
SuperScript™ IV VILO™ (SSIV VILO) 预混液是一种反应预混液,用于在RT-qPCR 中快速、灵敏且可重复地检测 cDNA 的合成。

SuperScript IV VILO 预混液特点:
•超快速10分钟 RT 反应
•超高产量—Ct 值较其他所有反转录试剂降低超过2个循环
•超便捷 单管 cDNA 反应预混液,可用于两步法 RT-qPCR
•超高效反应,对低模板量和纯度不佳的样本亦适用

凭借高持续合成能力、热稳定的 SuperScript™ IV 反转录酶及进一步优化的缓冲液,SSIV VILO 预混液将可靠的 VILO 技术提升至全新维度。这些成分可以在更高温度和更短时间内实现高效的 cDNA 合成。该预混液支持在更短的时间内以更高的温度高效地进行 cDNA 合成,即便纯度欠佳或模板有限的情况下,也能够获得出色的 cDNA 得率和灵敏度。SSIV VILO 预混液为您提供了一种全新的工具,可以实现更高效且可重复的 RT-qPCR。

为实现更好的性能,我们还提供了含 ezDNase 的 SuperScript IV VILO 预混液。加入ezDNase , 应用一个极其简化的基因组 DNA 去除步骤,可以进一步加快 RT-qPCR 工作流程。这显著缩短了整个反转录实验方案的时间,并减少了因常规 DNA 酶处理过程中 RNA 损失或损坏而导致的基因表达变化。

使用SSIV VILO 预混液,在更少的时间内您可以获得更多的 cDNA,加样更少,出现变化的可能更小,而且反应抑制减少,gDNA 干扰减少,使用的样品量也更少,这是其他 cDNA 合成试剂盒或预混液进行 RT-qPCR 所不能达到的。

来源
表达 M-MLV pol 基因的大肠杆菌产物的纯化,经过改良提高了热稳定性和半衰期、持续合成性、抑制剂耐受性,并降低了 RNase H 活性。

性能和质量测试
脱氧核糖核酸外切酶、脱氧核糖核酸外切酶及核糖核酸酶分析;cDNA 产物的产量和长度。
仅供科研使用。不可用于诊断程序。
规格
最终产品类型第一链 cDNA
产品规格管装
包括Kit with ezDnase
反应次数500 次反应
最佳反应温度50°C
数量Each
反应形式预混液
试剂类型反转录
逆转录酶SuperScript IV
核糖核酸酶 H 活性减少
运输条件干冰
尺寸(最终产品)长达 10 kb
原始材料RNA
技术反转录
最大浓度5X
检测方法qPCR
适用于(应用)RT-PCR
高 GC PCR 扩增效果
反应速度10 min。
Unit SizeEach
内容与储存

• SuperScript IV VILO 预混液 (2 x 1 mL)
• SuperScript IV VILO 预混液“无 RT”对照品 (1 mL)
• ezDNase 酶 (500 μL)
• 10X ezDNase 缓冲液 (1 mL)
• 无核酸酶水 (1.25 mL)

在 –30°C 至 –5°C 下储存。

常见问题解答 (FAQ)

Does the TaqMan Cells-to-CT Express Kit contain reagents for the removal of genomic DNA (gDNA)?

Yes, the TaqMan Cells-to-CT Express Kit includes Express ezDNase which can be added to the Express Lysis Solution for removal of gDNA during cell lysis. Express ezDNase is a double-strand specific, heat-labile DNase that will only digest gDNA, making it compatible with the downstream reverse transcription reaction. The enzyme is automatically inactivated during a heat kill step included in the reverse transcription program. To avoid the detection of gDNA, we recommend using a TaqMan Gene Expression Assay specifically designed to span an intron-exon boundary. Such assays are designated with an "_m1" suffix. More information can be found here.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

In your SuperScript IV RT protocols, there is no ezDNase inactivation step. Will active ezDNase affect RNA or the RT reaction?

The Invitrogen ezDNase Enzyme is a novel DNase that is highly specific for double-stranded DNA. It has no activity on single-stranded DNA in RT reactions (primers or probes), or on RNA. The enzyme is also thermolabile—it is inactivated quickly at temperatures typical for the SuperScript IV RT reaction (e.g., 50°C). The additional inactivation step is therefore not required in RT-qPCR applications.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Which SuperScript IV RT format do you recommend for real-time PCR applications?

For RT-qPCR applications we recommend using the Invitrogen SuperScript IV VILO Master Mix (Cat. No. 11756050). The cDNA synthesis reaction setup with this master mix requires fewer pipetting steps and therefore reduces variation in the data. SuperScript IV RT, as a component of the master mix, offers the highest efficiency of cDNA synthesis step compared to competitors’ products.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Are there any significant changes in the SuperScript IV RT protocol compared to the SuperScript III RT protocol?

The only change is that the incubation time for the reverse transcription reaction has been reduced from 50 minutes to 10 minutes. All the other parameters and steps are the same.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I get comparable cDNA yield and length using the SuperScript IV RT 10-minute protocol as when using the 50-minute protocol for SuperScript III RT?

When compared with SuperScript III RT (and other manufacturers’ RTs) in a synthesis reaction for a 9 kb cDNA, SuperScript IV RT performed successful synthesis in just 10 minutes and did so with comparable (or improved) yield (as shown by gel band density).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.