Construct design for efficient, effective and high-throughput gene silencing in plants.
AuthorsWesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, Waterhouse PM,
JournalPlant J
PubMed ID11576441
'Post-transcriptional silencing of plant genes using anti-sense or co-suppression constructs usually results in only a modest proportion of silenced individuals. Recent work has demonstrated the potential for constructs encoding self-complementary ''hairpin'' RNA (hpRNA) to efficiently silence genes. In this study we examine design rules for efficient gene silencing, in terms ... More
DNA cloning using in vitro site-specific recombination.
Authors Hartley J L; Temple G F; Brasch M A;
JournalGenome Res
PubMed ID11076863
As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More