EXPRESS qPCR Supermix, universal, 200 reactions - FAQs

I used the EXPRESS qPCR Supermix, universal and the PCR efficiency was below 90%. What is the problem?

This indicates that the PCR conditions were suboptimal. Please ensure that the reagents you are using have not been freeze-thawed multiple times and have not remained at room temperature for too long. Verify that the amount of primers you are using is correct.