预混 ROX 的 EXPRESS 一步法 SYBR™ GreenER™ 试剂盒
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预混 ROX 的 EXPRESS 一步法 SYBR™ GreenER™ 试剂盒

EXPRESS 一步法 SYBR GreenER 试剂盒与快速热循环仪兼容，适用于您的基于 SYBR 的 qPCR 实验。本试剂盒包含预混了解更多信息
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货号反应次数
1179001K
又称 11790-01K
2500 次反应
11790200500 次反应
货号 1179001K
又称 11790-01K
价格（CNY)
76,332.00
Each
添加至购物车
反应次数:
2500 次反应
价格（CNY)
76,332.00
Each
添加至购物车
EXPRESS 一步法 SYBR GreenER 试剂盒与快速热循环仪兼容，适用于您的基于 SYBR 的 qPCR 实验。本试剂盒包含预混 ROX 参比染料。还可提供包含一管单独的 ROX 参比染料的 EXPRESS 一步法 SYBR GreenER SuperMix 试剂盒。

此试剂盒提供：
•快速激活、抗体介导的 Platinum™ Taq DNA 聚合酶
•适用于一步法 qRT-PCR 的 一步法 SuperScript™ III 逆转录酶
• SYBR GreenER 染料
• UDG 残留污染控制—尿嘧啶-DNA-糖基化酶 (UDG)/dUTP 可减少残留污染

仅供科研使用。不可用于诊断程序。
规格
适用于（设备）7000系统、7300系统、7700系统、7900HT Fast 系统、7900HT 系统、Eppendorf™ MasterCycler™ RealPlex、StepOne™快速模式、StepOne™标准模式
产品规格管装
试剂盒类型EXPRESS 一步法 SYBR GreenER 试剂盒
反应次数2500 次反应
聚合酶Taq DNA 聚合酶
产品线SYBR™ GreenER™
产品类型实时荧光定量 PCR SYBR 预混液
数量2,500 reactions
样品类型RNA
足够用于2500 次反应
检测方法SYBR
适用于（应用）基因表达
PCR 方法一步法 RT-qPCR
反应速度快速，标准
Unit SizeEach
内容与储存
EXPRESS 一步法 SYBR™ GreenER™ SuperMix 试剂盒由2个模块组成：EXPRESS SYBR™ GreenER™ RT 模块（含有 SuperScript™ III 逆转录酶和 RNAseOUT™）和预混 ROX 的 SYBR™ GreenER™ qPCR SuperMix。

收到货以后请储存于 -20°C 条件下。初次解冻后，EXPRESS SYBR™ GreenER™ qPCR SuperMix 可在 +4·C 下储存长达1个月。妥善储存时，可以保证稳定6个月。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all 预混 ROX 的 EXPRESS 一步法 SYBR™ GreenER™ 试剂盒 FAQs