High-throughput yeast two-hybrid assays for large-scale protein interaction mapping.
AuthorsWalhout AJ, Vidal M,
JournalMethods
PubMed ID11403578
'Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we ... More
Functional identification of Arabidopsis stress regulatory genes using the controlled cDNA overexpression system.
AuthorsPapdi C, Abrahám E, Joseph MP, Popescu C, Koncz C, Szabados L,
JournalPlant Physiol
PubMed ID18441225
'Responses to environmental stresses in higher plants are controlled by a complex web of abscisic acid (ABA)-dependent and independent signaling pathways. To perform genetic screens for identification of novel Arabidopsis (Arabidopsis thaliana) loci involved in the control of abiotic stress responses, a complementary DNA (cDNA) expression library was created in ... More
Homologous high-throughput expression and purification of highly conserved E coli proteins.
AuthorsErgin A, Büssow K, Sieper J, Thiel A, Duchmann R, Adam T,
JournalMicrob Cell Fact
PubMed ID17553160
BACKGROUND: Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal intestinal flora is crucial for the development of intestinal inflammation. However, due to the complexity of the intestinal flora, it has been difficult to ... More
Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.
AuthorsGoshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R, Wakamatsu A, Yamamoto J, Kimura K, Nishikawa T, Andoh T, Iida Y, Ishikawa K, Ito E, Kagawa N, Kaminaga C, Kanehori K, Kawakami B, Kenmochi K, Kimura R, Kobayashi M, Kuroita T, Kuwayama H, Maruyama Y, Matsuo K, Minami K, Mitsubori M, Mori M, Morishita R, Murase A, Nishikawa A, Nishikawa S, Okamoto T, Sakagami N, Sakamoto Y, Sasaki Y, Seki T, Sono S, Sugiyama A, Sumiya T, Takayama T, Takayama Y, Takeda H, Togashi T, Yahata K, Yamada H,
JournalNat Methods
PubMed ID19054851
Appropriate resources and expression technology necessary for human proteomics on a whole-proteome scale are being developed. We prepared a foundation for simple and efficient production of human proteins using the versatile Gateway vector system. We generated 33,275 human Gateway entry clones for protein synthesis, developed mRNA expression protocols for them ... More