Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter.
AuthorsKamiyama S, Suda T, Ueda R, Suzuki M, Okubo R, Kikuchi N, Chiba Y, Goto S, Toyoda H, Saigo K, Watanabe M, Narimatsu H, Jigami Y, Nishihara S,
JournalJ Biol Chem
PubMed ID12716889
'Nucleotide sulfate, namely 3''-phosphoadenosine 5''-phosphosulfate (PAPS), is a universal sulfuryl donor for sulfation. Although a specific PAPS transporter is present in Golgi membrane, no study has reported the corresponding gene. We have identified a novel human gene encoding a PAPS transporter, which we have named PAPST1, and the Drosophila melanogaster ... More
SCAP ligands are potent new lipid-lowering drugs.
Authors Grand-Perret T; Bouillot A; Perrot A; Commans S; Walker M; Issandou M;
JournalNat Med
PubMed ID11726962
'Upregulation of low-density lipoprotein receptor (LDLr) is a key mechanism to control elevated plasma LDL-cholesterol levels. Here we identify a new class of compounds that directly binds to the sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP). We show that a 14C-labeled, photo-activatable analog specifically labeled both SCAP and a ... More
Characterization of tumor-associated Chk2 mutations.
AuthorsWu X, Webster SR, Chen J,
JournalJ Biol Chem
PubMed ID11053450
'The integrity of the DNA damage response pathway is essential for prevention of neoplastic transformation. Several proteins involved in this pathway including p53, BRCA1, and ATM are frequently mutated in human cancer. Checkpoint kinase 2 (Chk2) is a DNA damage-activated protein kinase that lies downstream of ATM in this pathway. ... More
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli.
'The steps involved in the biosynthesis of the ADP-L-glycero-beta-D-manno-heptose (ADP-L-beta-D-heptose) precursor of the inner core lipopolysaccharide (LPS) have not been completely elucidated. In this work, we have purified the enzymes involved in catalyzing the intermediate steps leading to the synthesis of ADP-D-beta-D-heptose and have biochemically characterized the reaction products by ... More
14-3-3 proteins mediate an essential anti-apoptotic signal.
Authors Masters S C; Fu H;
JournalJ Biol Chem
PubMed ID11577088
'The 14-3-3 proteins are a family of highly conserved eukaryotic regulatory molecules that play important roles in many biological processes including cell cycle control and regulation of cell death. They are able to carry out these effects through binding and modulating the activity of a host of signaling proteins. The ... More
Functional identification of Arabidopsis stress regulatory genes using the controlled cDNA overexpression system.
AuthorsPapdi C, Abrahám E, Joseph MP, Popescu C, Koncz C, Szabados L,
JournalPlant Physiol
PubMed ID18441225
'Responses to environmental stresses in higher plants are controlled by a complex web of abscisic acid (ABA)-dependent and independent signaling pathways. To perform genetic screens for identification of novel Arabidopsis (Arabidopsis thaliana) loci involved in the control of abiotic stress responses, a complementary DNA (cDNA) expression library was created in ... More
Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy.
Authors Gomes M D; Lecker S H; Jagoe R T; Navon A; Goldberg A L;
JournalProc Natl Acad Sci U S A
PubMed ID11717410
'Muscle wasting is a debilitating consequence of fasting, inactivity, cancer, and other systemic diseases that results primarily from accelerated protein degradation by the ubiquitin-proteasome pathway. To identify key factors in this process, we have used cDNA microarrays to compare normal and atrophying muscles and found a unique gene fragment that ... More
Identification of potential interaction networks using sequence-based searches for conserved protein-protein interactions or
Authors Matthews L R; Vaglio P; Reboul J; Ge H; Davis B P; Garrels J; Vincent S; Vidal M;
JournalGenome Res
PubMed ID11731503
Protein interaction maps have provided insight into the relationships among the predicted proteins of model organisms for which a genome sequence is available. These maps have been useful in generating potential interaction networks, which have confirmed the existence of known complexes and pathways and have suggested the existence of new ... More
Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion.
AuthorsYang HS, Matthews CP, Clair T, Wang Q, Baker AR, Li CC, Tan TH, Colburn NH,
JournalMol Cell Biol
PubMed ID16449643
Programmed cell death 4 (Pdcd4) suppresses neoplastic transformation by inhibiting the activation of c-Jun and consequently AP-1-dependent transcription. We report that Pdcd4 blocks c-Jun activation by inhibiting the expression of mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)/hematopoietic progenitor kinase 1, a kinase upstream of Jun N-terminal kinase (JNK). cDNA ... More
Drf1, a novel regulatory subunit for human Cdc7 kinase.
AuthorsMontagnoli A, Bosotti R, Villa F, Rialland M, Brotherton D, Mercurio C, Berthelsen J, Santocanale C,
JournalEMBO J
PubMed ID12065429
Studies in model organisms have contributed to elucidate multiple levels at which regulation of eukaryotic DNA replication occurs. Cdc7, an evolutionarily conserved serine-threonine kinase, plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Binding of the cell cycle-regulated ... More
Characterization of a heparan sulfate 3-O-sulfotransferase-5, an enzyme synthesizing a tetrasulfated disaccharide.
AuthorsMochizuki H, Yoshida K, Gotoh M, Sugioka S, Kikuchi N, Kwon YD, Tawada A, Maeyama K, Inaba N, Hiruma T, Kimata K, Narimatsu H,
JournalJ Biol Chem
PubMed ID12740361
Heparan sulfate d-glucosaminyl 3-O-sulfotransferases (3-OSTs) catalyze the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 3 of the glucosamine residue of heparan sulfate and heparin. A sixth member of the human 3-OST family, named 3-OST-5, was recently reported (Xia, G., Chen, J., Tiwari, V., Ju, W., Li, J.-P., Malmstrom, ... More
RtsA and RtsB Coordinately Regulate Expression of the Invasion and Flagellar Genes in Salmonella enterica Serovar Typhimurium.
AuthorsEllermeier CD, Slauch JM,
JournalJ Bacteriol
PubMed ID12923082
Salmonella enterica serovar Typhimurium encounters numerous host environments and defense mechanisms during the infection process. The bacterium responds by tightly regulating the expression of virulence genes. We identified two regulatory proteins, termed RtsA and RtsB, which are encoded in an operon located on an island integrated at tRNA(PheU) in S. ... More
Homologous high-throughput expression and purification of highly conserved E coli proteins.
AuthorsErgin A, Büssow K, Sieper J, Thiel A, Duchmann R, Adam T,
JournalMicrob Cell Fact
PubMed ID17553160
BACKGROUND: Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal intestinal flora is crucial for the development of intestinal inflammation. However, due to the complexity of the intestinal flora, it has been difficult to ... More
Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme.
AuthorsWang Y, Bruenn JA, Queener SF, Cody V,
JournalAntimicrob Agents Chemother
PubMed ID11502523
While assays of many antifolate inhibitors for dihydrofolate reductase (DHFR) have been performed using rat DHFR as a target, neither the sequence nor the structure of rat DHFR is known. Here, we report the isolation of the rat DHFR gene through screening of a rat liver cDNA library. The rat ... More
Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2.
AuthorsKamiyama S, Sasaki N, Goda E, Ui-Tei K, Saigo K, Narimatsu H, Jigami Y, Kannagi R, Irimura T, Nishihara S,
JournalJ Biol Chem
PubMed ID16492677
Sulfation is an important posttranslational modification associated with a variety of molecules. It requires the involvement of the high energy form of the universal sulfate donor, 3'-phosphoadenosine 5'-phosphosulfate (PAPS). Recently, we identified a PAPS transporter gene in both humans and Drosophila. Although human colonic epithelial tissues express many sulfated glycoconjugates, ... More
A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome.
Authors Davy A; Bello P; Thierry-Mieg N; Vaglio P; Hitti J; Doucette-Stamm L; Thierry-Mieg D; Reboul J; Boulton S; Walhout A J; Coux O; Vidal M;
JournalEMBO Rep
PubMed ID11559592
The ubiquitin-proteasome proteolytic pathway is pivotal in most biological processes. Despite a great level of information available for the eukaryotic 26S proteasome-the protease responsible for the degradation of ubiquitylated proteins-several structural and functional questions remain unanswered. To gain more insight into the assembly and function of the metazoan 26S proteasome, ... More
Biosynthesis of nucleotide-activated D-glycero-D-manno-heptose.
Authors Kneidinger B; Graninger M; Puchberger M; Kosma P; Messner P;
JournalJ Biol Chem
PubMed ID11279237
The glycan chain repeats of the S-layer glycoprotein of Aneurinibacillus thermoaerophilus DSM 10155 contain d-glycero-d-manno-heptose, which has also been described as constituent of lipopolysaccharide cores of Gram-negative bacteria. The four genes required for biosynthesis of the nucleotide-activated form GDP-d-glycero-d-manno-heptose were cloned, sequenced, and overexpressed in Escherichia coli, and the corresponding ... More
Mutations of the gene encoding the protein kinase A type I-alpha regulatory subunit in patients with the Carney complex.
Authors Kirschner L S; Carney J A; Pack S D; Taymans S E; Giatzakis C; Cho Y S; Cho-Chung Y S; Stratakis C A;
JournalNat Genet
PubMed ID10973256
Carney complex (CNC) is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumours and psammomatous melanotic schwannomas. CNC is inherited as an autosomal dominant trait and the genes responsible have been mapped to 2p16 and 17q22-24 (refs 6, 7). Because of its similarities to ... More
Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T.
Authors Kneidinger B; Graninger M; Adam G; Puchberger M; Kosma P; Zayni S; Messner P;
JournalJ Biol Chem
PubMed ID11096116
The glycan repeats of the surface layer glycoprotein of Aneurinibacillus thermoaerophilus L420-91T contain d-rhamnose and 3-acetamido-3,6-dideoxy-d-galactose, both of which are also constituents of lipopolysaccharides of Gram-negative plant and human pathogenic bacteria. The two genes required for biosynthesis of the nucleotide-activated precursor GDP-d-rhamnose, gmd and rmd, were cloned, sequenced, and overexpressed ... More
Molecular cloning and characterization of a novel UDP-GlcNAc:GalNAc-peptide beta1,3-N-acetylglucosaminyltransferase (beta 3Gn-T6), an enzyme synthesizing the core 3 structure of O-glycans.
The core 3 structure of the O-glycan, GlcNAcbeta1-3GalNAcalpha1-serine/threonine, an important precursor in the biosynthesis of mucin-type glycoproteins, is synthesized by UDP-N-acetylglucosamine:GalNAc-peptide beta1,3-N- acetylglucosaminyltransferase (beta3Gn-T; core 3 synthase). The core 3 structure is restricted in its occurrence to mucins from specific tissues such as the stomach, small intestine, and colon. A ... More
C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development.
Authors Koiwa Hisashi; Barb Adam W; Xiong Liming; Li Fang; McCully Michael G; Lee Byeong-Ha; Sokolchik Irina; Zhu Jianhua; Gong Zhizhong; Reddy Muppala; Sharkhuu Altanbadralt; Manabe Yuzuki; Yokoi Shuji; Zhu Jian-Kang; Bressan Ray A; Hasegawa Paul M;
JournalProc Natl Acad Sci U S A
PubMed ID12149434
Cold, hyperosmolarity, and abscisic acid (ABA) signaling induce RD29A expression, which is an indicator of the plant stress adaptation response. Two nonallelic Arabidopsis thaliana (ecotype C24) T-DNA insertional mutations, cpl1 and cpl3, were identified based on hyperinduction of RD29A expression that was monitored by using the luciferase (LUC) reporter gene ... More