SuperScript V VILO Master Mix, 1250 rxns

Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix is also available with ezDNase Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.

Features of SuperScript V VILO Master Mix include:

• Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
• Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
• Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
• Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
• Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility

SuperScript V VILO Master Mix combines SuperScript V RT, RNase inhibitor, buffer, dNTPs, and an optimized blend of oligo(dT) and random primers in a single formulation, simplifying reaction setup and helping reduce variability. Its combination of fast cDNA synthesis, robust performance, and flexible room-temperature handling makes it well suited for routine, high-throughput, and automated RT-qPCR workflows.

SuperScript V VILO Master Mix with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA, further simplifying the two-step RT-qPCR workflow.

A DTT-free, animal origin-free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.