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引用和文献 (25)
Invitrogen™
Gateway™ pDEST™17 载体
为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白了解更多信息
| 货号 | 数量 |
|---|---|
| 11803012 | 6 μg |
货号 11803012
价格（CNY)
5,723.20
飞享价
Ends: 31-Dec-2025
6,834.00共减 1,110.80 (16%)
Each
数量:
6 μg
价格（CNY)
5,723.20
飞享价
Ends: 31-Dec-2025
6,834.00共减 1,110.80 (16%)
Each
为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白。无论是入门克隆还是 Ultimate™ RF 克隆，所有 Gateway™ 目的载体具有使用任何 attL-侧区片段进行重组的 attR 位点。下表列出了一系列可用的目的载体。
所需的额外材料，可单独购买：
Gateway™ 入门克隆、Gateway™ LR Clonase™ 酶混合物和反应缓冲液。
所需的额外材料，可单独购买：
Gateway™ 入门克隆、Gateway™ LR Clonase™ 酶混合物和反应缓冲液。
仅供科研使用。不可用于诊断程序。
规格
耐抗生素细菌氨苄青霉素 (AmpR)
构成或诱导系统诱导
产品类型表达载体
数量6 μg
选择试剂（真核生物）无
载体pDEST、Gateway T7 载体
克隆方法Gateway™
产品线Gateway
促进剂T7
蛋白标记His 标签 (6x)
Unit SizeEach
内容与储存
pDEST™ 17 载体以 150ng/μl TE 缓冲液 (pH 8.0) 形式提供。在 -20°C 下储存。适当储存时可保证稳定 6 个月。
常见问题解答 (FAQ)
可以使用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶LR Clonase II酶进行BP/LR Clonase反应的一步法实验方案吗？
有推荐的一步式BP/LR重组实验方案吗？
如果丢失了入门克隆，如何将目的基因从一个Gateway兼容的表达克隆转移到一个新的目的载体？
我可以单独购买5X LR Clonase缓冲液或5X BP Clonase缓冲液吗？
是否提供用于在植物内表达的Gateway载体吗？
引用和文献 (25)
引用和文献
Abstract
Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.
Journal:J Biol Chem
PubMed ID:14699162
'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the
Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis.
Journal:J Biol Chem
PubMed ID:14715651
'Fucosylated glycoconjugates are involved in numerous biological events, and alpha-l-fucosidases, the enzymes responsible for their processing, are therefore of crucial importance. Deficiency in alpha-l-fucosidase activity is associated with fucosidosis, a lysosomal storage disorder characterized by rapid neurodegeneration, resulting in severe mental and motor deterioration. To gain insight into alpha-l-fucosidase function
The oncoprotein Tax binds the SRC-1-interacting domain of CBP/p300 to mediate transcriptional activation.
Journal:Mol Cell Biol
PubMed ID:11463834
'Oncogenesis associated with human T-cell leukemia virus (HTLV) infection is directly linked to the virally encoded transcription factor Tax. To activate HTLV-1 transcription Tax interacts with the cellular protein CREB and the pleiotropic coactivators CBP and p300. While extensively studied, the molecular mechanisms of Tax transcription function and coactivator utilization
Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol.
Journal:J Biol Chem
PubMed ID:16787914
'Human DNA polymerase N (POLN or pol nu) is the most recently discovered nuclear DNA polymerase in the human genome. It is an A-family DNA polymerase related to Escherichia coli pol I, human POLQ, and Drosophila Mus308. We report the first purification of the recombinant enzyme and examination of its
Dual-tagging system for the affinity purification of mammalian protein complexes.
Journal:Biotechniques
PubMed ID:17907572
'Although affinity purification coupled with mass spectrometry (MS) provides a powerful tool to study protein-protein interactions, this strategy has encountered numerous difficulties when adapted to mammalian cells. Here we describe a Gateway-compatible dual-tag affinity purification system that integrates regulatable expression, tetracysteine motifs, and various combinations ofaffinity tags to facilitate the