The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit.
AuthorsShimada M, Mahon MJ, Greer PA, Segre GV,
JournalEndocrinology
PubMed ID15691895
'We show calcium-dependent, direct binding between the N-terminal portion of the PTH/PTHrP receptor (PTH1R) C-terminal intracellular tail and the calpain small subunit. Binding requires, but may not be limited to, amino acids W474, S475, and W477. The wild-type, full-length rat (r) PTH1R, but not rPTH1R with W474A/W477A substitutions, copurifies with ... More
Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.
AuthorsKatagiri Y, Ingham KC.
JournalBiotechniques
PubMed ID12139250
All Six Modules of the Gelatin-binding Domain of Fibronectin Are Required for Full Affinity.
AuthorsKatagiri Y, Brew SA, Ingham KC,
JournalJ Biol Chem
PubMed ID12538576
The gelatin-binding sites of fibronectin are confined to a 42-kDa region having four type I and two type II modules in the following order: I(6)-II(1)-II(2)-I(7)-I(8)-I(9). To determine the relative importance of each module for recognition of gelatin, recombinant green fluorescent fusion proteins were prepared in which individual modules or groups ... More