A modified Gateway cloning strategy for overexpressing tagged proteins in plants.
AuthorsDubin MJ, Bowler C, Benvenuto G,
JournalPlant Methods
PubMed ID18211686
'ABSTRACT: BACKGROUND: Recent developments, including the sequencing of a number of plant genomes, have greatly increased the amount of data available to scientists and has enabled high throughput investigations where many genes are investigated simultaneously. To perform these studies, recombinational cloning methods such as the Gateway system have been adapted ... More
Trpm7 regulates cell adhesion by controlling the calcium dependent protease calpain.
AuthorsSu LT, Agapito MA, Li M, T N Simonson W, Huttenlocher A, Habas R, Yue L, Runnels LW,
JournalJ Biol Chem
PubMed ID16436382
'M-calpain is a protease implicated in the control of cell adhesion through focal adhesion disassembly. The mechanism by which the enzyme is spatially and temporally controlled is not well understood, particularly because calpain''s dependence on calcium exceeds the sub-micromolar concentrations normally observed in cells. Here we show that the channel-kinase ... More
High-throughput gateway bicistronic retroviral vectors for stable expression in mammalian cells: exploring the biologic effects of STAT5 overexpression.
AuthorsRoyer Y, Menu C, Liu X, Constantinescu SN,
JournalDNA Cell Biol
PubMed ID15231069
Stable expression of cloned genes in mammalian cells has been achieved in the past by retroviral transduction using bicistronic retroviral vectors. In these vectors, the use of an Internal Ribosome Entry Site (IRES) allows simultaneous expression of a protein of interest and a fluorescence marker. However, traditional cDNA cloning in ... More