Custom Primer - FAQs

查看更多产品信息 Custom Primer - FAQs (11846060)

5 个常见问题解答

What is the smallest quantity of RNA detectable by the SuperScript First-Strand System for RT-PCR?

Detection limits dependend on many factors, including primer design, target size, and the abundance of message. In our hands, this system was able to detect GAPDH mRNA from as little as 1.0 pg of total HeLa RNA when used in conjunction with Platinum Taq DNA Polymerase High Fidelity.

How does adding Platinum Taq DNA Polymerase improve SuperScript One-Step RT-PCR performance?

Platinum Taq DNA Polymerase is precomplexed with a mixture of antibodies that inhibit polymerase activity until the initial denaturation step in PCR. As a result, nonspecific polymerase acitivty at lower temperatures during set-up and reverse transcription is eliminated, which provides greater yield and specificity of intended product.

What size targets can be amplified by the SuperScript One-Step RT-PCR Systems?

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is recommended for amplifying RNA targets up to 4.5 kb and the SuperScript II One-Step RT-PCR System with Platinum Taq DNA Polymerase is recommended for amplifying RNA targets up to 3.5 kb. Even though the SuperScript II and III One-Step RT-PCR Systems with Platinum Taq High Fidelity can amplify smaller targets, it is recommended for amplifying RNA targets from 1 kb up to 9 kb.

How stable are your cationic lipids that are used in transfection?

All the following lipids are stable at 4º C for at least one year:
11668-019 Lipofectamine 2000
10362-100 Cellfectin II
10459-014 DMRIE-C
10964-013 Lipofectamine PLUS
18292-011 Lipofectin
18324-012 Lipofectamine

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I sequence a peptide that is acetylated or biotinylated?

No. These groups effectively block the N terminus.