SuperScript™ Double-Stranded cDNA Synthesis Kit, 50 Reactions - FAQs

查看更多产品信息 SuperScript™ Double-Stranded cDNA Synthesis Kit - FAQs (11917020, 11917010)

3 个常见问题解答

我想从mRNA生成双链DNA(dsDNA)，贵公司有适合的试剂盒吗？

当然，为此我们提供了三种试剂盒：

1.SuperScript Plasmid System，适用于cDNA合成和克隆 ◦可生成cDNA并将其克隆到含有CMV启动子、兼容Gateway的载体(pCMVSport6)之中，以便进行哺乳动物细胞表达筛选
◦实验需要mRNA（必须具有poly(A)尾）作为起始材料
◦可以在大肠杆菌中进行探针筛查，但不可进行表达筛查
◦产生单向、包含Sal I / Not I酶切位点的dsDNA

2.SuperScript Choice系统，适用于cDNA合成 ◦适用于任何RNA来源
◦可以使用随机引物、oligo(dT)引物或混合引物
◦会产生带有EcoRI末端的cDNA，可以克隆到任何具有EcoRI 位点的载体（质粒或噬菌体）中
◦有益于生成cDNA文库
◦产生的dsDNA可双向克隆

3.SuperScript双链cDNA合成试剂盒 ◦可用于生成平端、双链cDNA
◦可使用总RNA或mRNA作为起始材料

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

I'd like to generate double-stranded DNA (dsDNA) from mRNA. Do you have a kit for this?

1.SuperScript Plasmid System for cDNA Synthesis and Cloning
-Generating and cloning cDNA into a CMV-containing Gateway-compatible vector (pCMVSport6) for mammalian screening
-mRNA required as starting material (must have poly(A) tail)
-Can probe screen in E.coli but not expression screen
-Resulting dsDNA is unidirectional with Sal I / Not I sites

2.SuperScript Choice System for cDNA Synthesis
-Will work on any RNA source
-Can use random, oligo(dT), or combination
-Will result in cDNA with EcoRI ends which can be cloned into any vector (plasmid or phage) that has EcoRI sites
-Good for generation of cDNA libraries
-Resulting dsDNA will clone bidirectionally

3.SuperScript Double-Stranded cDNA Synthesis Kit
-For generating blunt-ended, double-stranded cDNA
-Will work for total RNA or mRNA as starting material