Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection.
AuthorsMier NC,Roper DK
JournalPloS one
PubMed ID38833479
Lentiviral vectors derived from human immunodeficiency virus type I are widely used to deliver functional gene copies to mammalian cells for research and gene therapies. Post-transcriptional splicing of lentiviral vector transgene in transduced host and transfected producer cells presents barriers to widespread application of lentiviral vector-based therapies. The present study ... More
Fibroblast growth factor receptor-1 signaling induces osteopontin expression and vascular smooth muscle cell-dependent adventitial fibroblast migration in vitro.
Authors Li Guohong; Oparil Suzanne; Kelpke Stacey S; Chen Yiu-Fai; Thompson John A;
JournalCirculation
PubMed ID12176960
'BACKGROUND: Increased expression of osteopontin (OPN), fibroblast growth factors (FGFs), and their type-1 receptor (FGFR-1) is associated with neointima formation and atherosclerosis. This study tested the hypothesis that ligand activation of FGFR-1 stimulates OPN expression in rat aortic smooth muscle cells (RASMCs), explored the signaling pathway involved, and assessed the ... More
Derivation of human embryonic stem cell lines from vitrified human embryos.
AuthorsPeura TT, Schaft J, Stojanov T
JournalMethods Mol Biol
PubMed ID19907970
'Human embryonic stem cell lines are usually derived from human embryos that have become excess to clinical needs in assisted reproduction programs, whether because the couple in question has completed their family or because the embryo was found to be clinically unsuitable for transfer due to severe genetic condition (in ... More
Insulin and insulin-like growth factor I receptors utilize different G protein signaling components.
We examined the role of heterotrimeric G protein signaling components in insulin and insulin-like growth factor I (IGF-I) action. In HIRcB cells and in 3T3L1 adipocytes, treatment with the Galpha(i) inhibitor (pertussis toxin) or microinjection of the Gbetagamma inhibitor (glutathione S-transferase-betaARK) inhibited IGF-I and lysophosphatidic acid-stimulated mitogenesis but had no ... More
Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival.
Authors Sotiriou Sotiria; Gispert Suzana; Cheng Jun; Wang Yaohui; Chen Amy; Hoogstraten-Miller Shelley; Miller Georgina F; Kwon Oran; Levine Mark; Guttentag Susan H; Nussbaum Robert L;
JournalNat Med
PubMed ID11984580
The only proven requirement for ascorbic acid (vitamin C) is in preventing scurvy, presumably because it is a cofactor for hydroxylases required for post-translational modifications that stabilize collagen. We have created mice deficient in the mouse ortholog (solute carrier family 23 member 1 or Slc23a1) of a rat ascorbic-acid transporter, ... More
Neoplastic transformation induced by insulin receptor substrate-1 overexpression requires an interaction with both Grb2 and Syp signaling molecules.
Authors Tanaka S; Ito T; Wands J R;
JournalJ Biol Chem
PubMed ID8662827
The insulin receptor substrate-1 (IRS-1) is the major intracellular substrate of insulin and insulin-like growth factor-I (IGF-I) receptor tyrosine kinase activity, and this protein has been found to be overexpressed in human hepatocellular carcinomas. IRS-1 contains several src homology 2 (SH2) binding motifs that interact following tyrosyl phosphorylation with SH2-containing ... More
Molecular cloning and functional characterization of murine sphingosine kinase.
Authors Kohama T; Olivera A; Edsall L; Nagiec M M; Dickson R; Spiegel S;
JournalJ Biol Chem
PubMed ID9726979
Sphingosine-1-phosphate (SPP) is a novel lipid messenger that has dual function. Intracellularly it regulates proliferation and survival, and extracellularly, it is a ligand for the G protein-coupled receptor Edg-1. Based on peptide sequences obtained from purified rat kidney sphingosine kinase, the enzyme that regulates SPP levels, we report here the ... More
Mutation of a conserved amino acid residue (tryptophan 1173) in the tyrosine kinase domain of the IGF-I receptor abolishes autophosphorylation but does not eliminate biologic function.
Authors Blakesley V A; Kato H; Roberts C T Jr; LeRoith D;
JournalJ Biol Chem
PubMed ID7852347
The amino acid sequence of the tyrosine kinase domain of the insulin-like growth factor-I (IGF-I) receptor is 84% identical to the sequence of the analogous region of the insulin receptor. A naturally occurring mutation of the tryptophan residue at position 1200 of the insulin receptor to serine results in impaired ... More
Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.
Authors Benbow U; Butticè G; Nagase H; Kurkinen M;
JournalJ Biol Chem
PubMed ID8631880
The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J., ... More
Statin prevents tissue factor expression in human endothelial cells: role of Rho/Rho-kinase and Akt pathways.
Authors Eto Masato; Kozai Toshiyuki; Cosentino Francesco; Joch Hana; Lüscher Thomas F;
JournalCirculation
PubMed ID11956113
BACKGROUND: Tissue factor plays a pivotal role in thrombus formation in acute coronary syndromes. However, the regulatory mechanisms underlying tissue factor expression are poorly understood. Statins are effective in patients with acute coronary syndromes. Hence, the aim of this study was to clarify in human endothelial cells the signaling pathways ... More