Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-a.
AuthorsHoulihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, Okano H, Matsuzaki Y
JournalNat Protoc
PubMed ID23154782
Platelet-derived growth factor receptor a (PDGFR-a) and stem cell antigen 1 (Sca-1) have recently been identified as selective markers of mouse mesenchymal stem cells (MSCs). PDGFR-a(+)Sca-1(+) (PaS) MSCs have augmented growth potential and robust tri-lineage differentiation compared with standard culture-selected MSCs. In addition, the selective isolation of PaS MSCs avoids ... More