BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP).
AuthorsSu Hua Poo; Nakada-Tsukui Kumiko; Tosello-Trampont Annie-Carole; Li Yonghe; Bu Guojun; Henson Peter M; Ravichandran Kodimangalam S;
JournalJ Biol Chem
PubMed ID11729193
The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, as well as inflammation and autoimmunity. The molecular details of the engulfment of apoptotic cells are not fully understood. ced-6 and its human homologue gulp, encode an adapter protein, whose function in engulfment is highly ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
AuthorsBonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
JournalExperimental cell research
PubMed ID38499143
Cathepsin L and cathepsin B mediate reovirus disassembly in murine fibroblast cells.
'After attachment to receptors, reovirus virions are internalized by endocytosis and exposed to acid-dependent proteases that catalyze viral disassembly. Previous studies using the cysteine protease inhibitor E64 and a mutant cell line that does not support reovirus disassembly suggest a requirement for specific endocytic proteases in reovirus entry. This study ... More
G alpha 13 stimulates Na+-H+ exchange through distinct Cdc42-dependent and RhoA-dependent pathways.
Authors Hooley R; Yu C Y; Symons M; Barber D L;
JournalJ Biol Chem
PubMed ID8626403
'Activity of the ubiquitously expressed Na+-H+ exchanger subtype NHE1 is stimulated upon activation of receptor tyrosine kinases and G protein-coupled receptors. The intracellular signaling pathways mediating receptor regulation of the exchanger, however, are poorly understood. Using transient expression of dominant interfering and constitutively active alleles in CCL39 fibroblasts, we determined ... More
Hepatocyte growth factor/scatter factor binds to small heparin-derived oligosaccharides and stimulates the proliferation of human HaCaT keratinocytes.
'Hepatocyte growth factor/scatter factor (HGF/SF) acts via a dual receptor system consisting of the MET tyrosine kinase receptor and heparan sulfate or dermatan sulfate proteoglycans. In optical biosensor binding assays, competition by oligosaccharides for binding of HGF/SF to immobilized heparin showed that disaccharides failed to compete, whereas tetrasaccharides inhibited HGF/SF ... More
Ca2+ inhibition of type III adenylyl cyclase in vivo.
Authors Wayman G A; Impey S; Storm D R;
JournalJ Biol Chem
PubMed ID7665559
'Type III adenylyl cyclase is stimulated by beta-adrenergic agonists and glucagon in vitro and in vivo, but not by Ca2+ and calmodulin. However, the enzyme is stimulated by Ca2+ and calmodulin in vitro when it is concomitantly activated by the guanyl nucleotide stimulatory protein Gs (Choi, E. J., Xia, Z., ... More
Metabolism of 4 beta -hydroxycholesterol in humans.
'One of the major oxysterols in the human circulation is 4 beta-hydroxycholesterol formed from cholesterol by the drug-metabolizing enzyme cytochrome P450 3A4. Deuterium-labeled 4 beta-hydroxycholesterol was injected into two healthy volunteers, and the apparent half-life was found to be 64 and 60 h, respectively. We have determined earlier the half-lives ... More
Activation of retinoic acid receptor-dependent transcription by all-trans-retinoic acid metabolites and isomers.
Authors Idres Nadia; Marill Julie; Flexor Maria A; Chabot Guy G;
JournalJ Biol Chem
PubMed ID12070176
'We have shown that four metabolites of all-trans-retinoic acid (ATRA) (4-oxo-, 4-OH-, 18-OH-, and 5,6-epoxy-RA) can induce maturation of NB4 promyelocytic leukemia cells (Idres, N., Benoit, G., Flexor, M. A., Lanotte, M., and Chabot, G. G. (2001) Cancer Res. 61, 700-705). To better understand the mechanism of action of ATRA ... More
Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity.
Authors Bies Juraj; Markus Ján; Wolff Linda;
JournalJ Biol Chem
PubMed ID11779867
'The transcription factor c-Myb is subject to several types of post-translational modifications, including phosphorylation, acetylation, and ubiquitination. These modifications regulate the transcription and transforming activity as well as the proteolytic stability of c-Myb. Here we report the covalent modification of c-Myb with the small ubiquitin-related protein SUMO-1. Mutational analysis identified ... More
The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF.
Authors Laura Richard P; Witt Andrea S; Held Heike A; Gerstner Resi; Deshayes Kurt; Koehler Michael F T; Kosik Kenneth S; Sidhu Sachdev S; Lasky Laurence A;
JournalJ Biol Chem
PubMed ID11821434
'Erbin is a recently described member of the LAP (leucine-rich repeat and PDZ domain) protein family. We used a C-terminally displayed phage peptide library to identify optimal ligands for the Erbin PDZ domain. Phage-selected peptides were type 1 PDZ ligands that bound with high affinity and specificity to the Erbin ... More
Transcriptional down-regulation of poly(ADP-ribose) polymerase gene expression by E1A binding to pRb proteins protects murine keratinocytes from radiation-induced apoptosis.
Authors Pacini A; Quattrone A; Denegri M; Fiorillo C; Nediani C; Ramon y Cajal S; Nassi P;
JournalJ Biol Chem
PubMed ID10574992
'Adenovirus E1A confers enhanced cell sensitivity to radiation and drug-induced DNA damage by a mechanism involving the binding to cellular proteins. Mutant analysis in E1A-transfected murine keratinocytes demonstrates that increased sensitivity to DNA damage requires at least E1A binding to the p300/CREB-binding protein (CBP) transcriptional coactivators and to pRb family ... More
Molecular cloning and functional analysis of the promoter of the human squalene synthase gene.
Authors Guan G; Jiang G; Koch R L; Shechter I;
JournalJ Biol Chem
PubMed ID7665618
'We have cloned and characterized the 5''-flanking region of the gene encoding human squalene synthase. We report here the promoter activity of successively 5''-truncated sections of a 1 kilobase of this region by fusing it to the coding region of a luciferase reporter gene. DNA segments of 200 base pairs ... More
Different composite regulatory elements direct expression of the human alpha subunit gene to pituitary and placenta.
Authors Heckert L L; Schultz K; Nilson J H;
JournalJ Biol Chem
PubMed ID7592867
'To identify elements of the human alpha subunit gene necessary for cell-specific expression, we generated an array of block mutations spanning approximately 400 base pairs (bp) of promoter proximal region and examined them using transient transfection analysis in pituitary (alpha T3) and placental (BeWo) cell lines. Comparison of promoter activity ... More
A ligand-inducible epidermal growth factor receptor/anaplastic lymphoma kinase chimera promotes mitogenesis and transforming properties in 3T3 cells.
'Oncogenic rearrangements of the anaplastic lymphoma kinase (ALK) gene, encoding a receptor type tyrosine kinase, are frequently associated with anaplastic large cell lymphomas. Such rearrangements juxtapose the intracellular domain of ALK to 5''-end sequences belonging to different genes and create transforming fusion proteins. To understand how the oncogenic versions of ... More
Inhibition of transforming growth factor beta signaling and Smad-dependent activation of transcription by the Latent Membrane Protein 1 of Epstein-Barr virus.
'Inhibition of transforming growth factor beta (TGFbeta) signaling by the Epstein-Barr virus Latent Membrane Protein 1 (LMP1) may account, at least in part, for the oncogenic activity of LMP1. We found that LMP1 is a potent inhibitor of TGFbeta signaling and Smad-dependent activation of transcription in 293 epithelial cells and ... More
Tissue-specific expression of the nonneuronal promoter of the aromatic L-amino acid decarboxylase gene is regulated by hepatocyte nuclear factor 1.
Authors Aguanno A; Afar R; Albert V R;
JournalJ Biol Chem
PubMed ID8626808
'The rat aromatic l-amino acid decarboxylase (AADC) gene contains alternative promoters which direct expression of neuronal and nonneuronal mRNAs that differ only in their 5''-untranslated regions (UTRs). We have analyzed the expression of the nonneuronal promoter of the rat AADC gene in the kidney epithelial cell line LLC-PK1 and in ... More
Efficacy of acetaminophen in skin B16-F0 melanoma tumor-bearing C57BL/6 mice.
AuthorsVad NM, Kudugunti SK, Graber D, Bailey N, Srivenugopal K, Moridani MY
JournalInt J Oncol
PubMed ID19513568
'Previously, we reported that acetaminophen (APAP) showed selective toxicity towards melanoma cell lines. In the current study, we investigated further the role of tyrosinase in APAP toxicity in SK-MEL-28 melanoma cells in the presence of a short hairpin RNA (shRNA) plasmid, silencing tyrosinase gene. Results from tyrosinase shRNA experiments showed ... More
A Principal Role for the Proteasome in Endoplasmic Reticulum-associated Degradation of Misfolded Intracellular Cystic Fibrosis Transmembrane Conductance Regulator.
Authors Gelman Marina S; Kannegaard Elisa S; Kopito Ron R;
JournalJ Biol Chem
PubMed ID11812794
'Endoplasmic reticulum-associated degradation of misfolded cystic fibrosis transmembrane conductance regulator (CFTR) protein is known to involve the ubiquitin-proteasome system. In addition, an ATP-independent proteolytic system has been suggested to operate in parallel with this pathway and become up-regulated when proteasomes are inhibited (Jensen, T. J., Loo, M. A., Pind, S., ... More
Comparison of the intracellular signaling responses by three chimeric fibroblast growth factor receptors in PC12 cells.
Authors Raffioni S; Thomas D; Foehr E D; Thompson L M; Bradshaw R A;
JournalProc Natl Acad Sci U S A
PubMed ID10377388
'Stably transfected PC12 cell lines expressing similar amounts of chimeric receptors composed of the extracellular domain of the human platelet-derived growth factor (PDGF)beta receptor and the transmembrane and intracellular domains of the fibroblast growth factor receptors (FGFRs) 1, 3, and 4 undergo ligand-induced differentiation. The FGFR1 chimera (PFR1) is the ... More
beta 1-Integrin-mediated Glioma Cell Adhesion and Free Radical-induced Apoptosis Are Regulated by Binding to a C-terminal Domain of PG-M/Versican.
Authors Wu Yaojiong; Chen Liwen; Zheng Peng-Sheng; Yang Burton B;
JournalJ Biol Chem
PubMed ID11805102
'Integrins are cell-surface glycoproteins that mediate cell activities, including tissue morphogenesis, development, immune response, and cancer, through interaction with extracellular proteins. Here we report a novel means by which integrin signaling and functions are regulated. In pull-down assays and immunoprecipitation, beta(1)-integrin bound to the C-terminal domain of PG-M/versican, an extracellular ... More
Alix (ALG-2-interacting protein X), a protein involved in apoptosis, binds to endophilins and induces cytoplasmic vacuolization.
'ALG-2-interacting protein X (Alix), also known as AIP1, is a cytoplasmic protein ubiquitously expressed and concentrated in phagosomes and exosomes. Alix may regulate apoptosis since it binds apoptosis-linked gene 2 (ALG-2), a Ca2+-binding protein necessary for cell death, and also overexpression of its C-terminal half (Alix-CT) blocks death induced by ... More
A novel function of BCL-2 overexpression in regulatory volume decrease. Enhancing swelling-activated Ca(2+) entry and Cl(-) channel activity.
Authors Shen Meng-Ru; Yang Tzi-Peng; Tang Ming-Jer;
JournalJ Biol Chem
PubMed ID11861644
'The cellular function of the oncogene bcl-2, a key regulator of apoptosis, is still debated. The goal of this study was to explore the relationship between BCL-2 overexpression and cell volume regulation by using two independent models, Madin-Darby canine kidney (MDCK) cells stably transfected with BCL-2 and MDCK clones with ... More
Simulated microgravity culture system for a 3-D carcinoma tissue model.
AuthorsNakamura K, Kuga H, Morisaki T, Baba E, Sato N, Mizumoto K, Sueishi K, Tanaka M, Katano M,
JournalBiotechniques
PubMed ID12449385
'An in vitro organotypic culture model is needed to understand the complexities of carcinoma tissue consisting of carcinoma cells, stromal cells, and extracellular matrices. We developed a new in vitro model of carcinoma tissue using a rotary cell culture system with four disposable vessels (RCCS-4D) that provides a simulated microgravity ... More
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
AuthorsFriedrich U, Blom AM, Dahlbäck B, Villoutreix BO,
JournalJ Biol Chem
PubMed ID11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More
Growth-dependent Regulation of Mammalian Pyrimidine Biosynthesis by the Protein Kinase A and MAPK Signaling Cascades.
Authors Sigoillot Frederic D; Evans David R; Guy Hedeel I;
JournalJ Biol Chem
PubMed ID11872754
'The carbamoyl phosphate synthetase domain of the multifunctional protein CAD catalyzes the initial, rate-limiting step in mammalian de novo pyrimidine biosynthesis. In addition to allosteric regulation by the inhibitor UTP and the activator PRPP, the carbamoyl phosphate synthetase activity is controlled by mitogen-activated protein kinase (MAPK)- and protein kinase A ... More
Mitogen-activated protein kinases and activator protein 1 are required for proliferation and cardiomyocyte differentiation of P19 embryonal carcinoma cells.
Authors Eriksson Minna; Leppä Sirpa;
JournalJ Biol Chem
PubMed ID11884386
'Mitogen-activated protein kinases (MAPKs) have been implicated as regulators of differentiation. The biological effect of MAPK signaling in the nucleus is achieved by signal-responsive transcription factors. Here we have investigated MAPK signaling and activation of AP-1 transcription factors in P19 embryonal carcinoma cells undergoing cardiomyocyte differentiation. We show that aggregation ... More
Authors Lin X; Voyno-Yasenetskaya T A; Hooley R; Lin C Y; Orlowski J; Barber D L;
JournalJ Biol Chem
PubMed ID8798430
'Activation of several GTPases stimulates Na+-H+ exchange, resulting in an increased efflux of intracellular H+. These GTPases include alpha subunits of the heterotrimeric G proteins Gq and G13, as well as the low molecular weight GTP-binding proteins Ras, Cdc42, and Rho (Hooley, R., Yu, C.-Y., Simon, M., and Barber, D. ... More
Hypoxia Sensitizes Cells to Nitric Oxide-induced Apoptosis.
Authors Lee Vivian Y; McClintock David S; Santore Matthew T; Budinger G R Scott; Chandel Navdeep S;
JournalJ Biol Chem
PubMed ID11861645
'Nitric oxide (NO) can induce apoptosis in a variety of cell types. A non-toxic concentration of nitric oxide under normal oxygen conditions triggered cell death under hypoxic conditions (1.5% O(2)) in fibroblasts. Nitric oxide administered during hypoxia induced the release of cytochrome c, caspase-9 activation, and the loss of mitochondrial ... More
Agonist-induced internalization of the platelet-activating factor receptor is dependent on arrestins but independent of G-protein activation. Role of the C terminus and the (D/N)PXXY motif.
'As with most G-protein-coupled receptors, repeated agonist stimulation of the platelet-activating factor receptor (PAFR) results in its desensitization, sequestration, and internalization. In this report, we show that agonist-induced PAFR internalization is independent of G-protein activation but is dependent on arrestins and involves the interaction of arrestins with a limited region ... More
Interactions between protein kinase CK2 and Pin1. Evidence for phosphorylation-dependent interactions.
Authors Messenger Moira M; Saulnier Ronald B; Gilchrist Andrew D; Diamond Phaedra; Gorbsky Gary J; Litchfield David W;
JournalJ Biol Chem
PubMed ID11940573
'The peptidyl-prolyl isomerase Pin1 interacts in a phosphorylation-dependent manner with several proteins involved in cell cycle events. In this study, we demonstrate that Pin1 interacts with protein kinase CK2, an enzyme that generally exists in tetrameric complexes composed of two catalytic CK2 alpha and/or CK2 alpha'' subunits together with two ... More
Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells.
'Abnormal vascular smooth muscle cell (VSMC) proliferation is a key feature of atherosclerosis and restenosis; however, the mechanisms regulating growth remain unclear. Herein we show that inhibition of the aldehyde-metabolizing enzyme aldose reductase (AR) inhibits NF-kappa B activation during restenosis of balloon-injured rat carotid arteries as well as VSMC proliferation ... More
A novel site on the Galpha -protein that recognizes heptahelical receptors.
'Specific domains of the G-protein alpha subunit have been shown to control coupling to heptahelical receptors. The extreme N and C termini and a region between alpha4 and alpha5 helices of the G-protein alpha subunit are known to determine selective interaction with the receptors. The metabotropic glutamate receptor 2 activated ... More
The biosynthesis of neurotrophin heterodimers by transfected mammalian cells.
Authors Heymach J V Jr; Shooter E M;
JournalJ Biol Chem
PubMed ID7744882
'Prompted by the recent discovery that neurotrophins, which are known to be biologically active as noncovalently linked homodimers, can also be induced to form biologically active heterodimers in vitro, we have investigated the biosynthesis of neurotrophin heterodimers by transfected mammalian cells. When COS cells were cotransfected with expression plasmids for ... More
Distinct residues in the carboxyl tail mediate agonist-induced desensitization and internalization of the human dopamine D1 receptor.
Authors Lamey Michael; Thompson Miles; Varghese George; Chi Hong; Sawzdargo Marek; George Susan R; O'Dowd Brian F;
JournalJ Biol Chem
PubMed ID11773080
'We have shown in a previous study that desensitization and internalization of the human dopamine D(1) receptor following short-term agonist exposure are mediated by temporally and biochemically distinct mechanisms. In the present study, we have used site-directed mutagenesis to remove potential phosphorylation sites in the third intracellular loop and carboxyl ... More
Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex.
'The survival of motor neurons (SMN) protein is the product of the gene mutated or deleted in the neurodegenerative disease, spinal muscular atrophy. SMN is part of a large macromolecular complex that also contains Gemin2, Gemin3, Gemin4, Gemin5, and Gemin6. The SMN complex functions in the assembly of spliceosomal small ... More
The human papillomavirus oncoprotein E7 attenuates NF-kappa B activation by targeting the Ikappa B kinase complex.
Authors Spitkovsky Dimitry; Hehner Steffen P; Hofmann Thomas G; Möller Andreas; Schmitz M Lienhard;
JournalJ Biol Chem
PubMed ID11986318
'Infection with high-risk human papillomaviruses (HPV) can lead to the development of cervical carcinomas. This process critically depends on the virus-encoded E6 and E7 oncoproteins, which stimulate proliferation by manipulating the function of a variety of host key regulatory proteins. Here we show that both viral proteins dose-dependently interfere with ... More
Maintenance of Integrated Proviral Gene Expression Requires Brm, a Catalytic Subunit of SWI/SNF Complex.
Authors Mizutani Taketoshi; Ito Taiji; Nishina Mitsue; Yamamichi Nobutake; Watanabe Akiko; Iba Hideo;
JournalJ Biol Chem
PubMed ID11850427
'We show here that murine leukemia virus-based retrovirus vector transgene expression is rapidly silenced in human tumor cell lines lacking expression of Brm, a catalytic subunit of the SWI/SNF chromatin remodeling complex, even though these vectors can successfully enter, integrate, and initiate transcription. We detected this gene silencing as a ... More
Changes in Mobility Account for Camptothecin-induced Subnuclear Relocation of Topoisomerase I.
Authors Christensen Morten O; Barthelmes Hans U; Feineis Silke; Knudsen Birgitta R; Andersen Anni H; Boege Fritz; Mielke Christian;
JournalJ Biol Chem
PubMed ID11907023
'DNA topoisomerase I is a nucleolar protein, which relocates to the nucleoplasm in response to drugs stabilizing topoisomerase I.DNA intermediates (e.g. camptothecin). Here we demonstrate that this phenomenon is solely caused by the drug''s impact on the interplay between mobility and localization of topoisomerase I in a living cell nucleus. ... More
Salt-inducible Kinase Represses cAMP-dependent Protein Kinase-mediated Activation of Human Cholesterol Side Chain Cleavage Cytochrome P450 Promoter through the CREB Basic Leucine Zipper Domain.
Authors Doi Junko; Takemori Hiroshi; Lin Xing-Zi; Horike Nanao; Katoh Yoshiko; Okamoto Mitsuhiro;
JournalJ Biol Chem
PubMed ID11864972
'Salt-inducible kinase (SIK), one of the serine/threonine protein kinases, was transiently expressed in Y1 cells during the early phase of the ACTH/cAMP-dependent protein kinase (PKA)-mediated signal transduction. The overexpression of SIK(N), the SIK''s N-terminal kinase domain, repressed the expression of the side chain cleavage cytochrome P450 (CYP11A) gene. To elucidate ... More
Authors Wilcox-Adelman Sarah A; Denhez Fabienne; Goetinck Paul F;
JournalJ Biol Chem
PubMed ID12087088
'The cell-surface heparan sulfate proteoglycan syndecan-4 acts in conjunction with the alpha(5)beta(1) integrin to promote the formation of actin stress fibers and focal adhesions in fibronectin (FN)-adherent cells. Fibroblasts seeded onto the cell-binding domain (CBD) fragment of FN attach but do not fully spread or form focal adhesions. Activation of ... More
Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling.
AuthorsUtani A, Momota Y, Endo H, Kasuya Y, Beck K, Suzuki N, Nomizu M, Shinkai H,
JournalJ Biol Chem
PubMed ID12826666
'The LG4 module of the laminin alpha 3 chain (alpha 3 LG4), a component of epithelial-specific laminin-5, has cell attachment activity and binds syndecan (Utani, A., Nomizu, M., Matsuura, H., Kato, K., Kobayashi, T., Takeda, U., Aota, S., Nielsen, P. K., and Shinkai, H. (2001) J. Biol. Chem. 276, 28779-28788). ... More
Interleukin 24 (MDA-7/MOB-5) signals through two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2.
Authors Wang Mai; Tan Zhongjia; Zhang Rong; Kotenko Sergei V; Liang Peng;
JournalJ Biol Chem
PubMed ID11706020
'Interleukin 24 (IL-24) encodes a secreted protein that exhibits significant homology to the interleukin 10 (IL-10) family of cytokines. Here we show that the human IL-24 is secreted by activated peripheral blood mononuclear cells and is the ligand for two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. The latter is also the ... More
Foam cell formation inhibits growth of Chlamydia pneumoniae but does not attenuate Chlamydia pneumoniae-induced secretion of proinflammatory cytokines.
Authors Blessing Erwin; Kuo Cho-Chou; Lin Tsun-Mei; Campbell Lee Ann; Bea Florian; Chesebro Brian; Rosenfeld Michael E;
JournalCirculation
PubMed ID11997286
'BACKGROUND: It has not yet been determined whether lipid-loaded macrophages (foam cells), a major cellular component of atherosclerotic lesions, have the capacity to support growth of Chlamydia pneumoniae and be activated to secrete proinflammatory cytokines in response to C pneumoniae infection. METHODS AND RESULTS: Lipid loading of RAW 264.7 cells ... More
The potent anti-HIV protein cyanovirin-N contains two novel carbohydrate binding sites that selectively bind to Man(8) D1D3 and Man(9) with nanomolar affinity: implications for binding to the HIV envelope protein gp120.
Authors Bewley C A; Otero-Quintero S
JournalJ Am Chem Soc
PubMed ID11457139
'Cyanovirin-N (CVN) is a monomeric 11 kDa cyanobacterial protein that potently inactivates diverse strains of human immunodeficiency virus (HIV) at the level of cell fusion by virtue of high affinity interactions with the surface envelope glycoprotein gp120. Several lines of evidence have suggested that CVN-gp120 interactions are in part mediated ... More
A yersinia effector and a pseudomonas avirulence protein define a family of cysteine proteases functioning in bacterial pathogenesis.
Authors Shao Feng; Merritt Peter M; Bao Zhaoqin; Innes Roger W; Dixon Jack E;
JournalCell
PubMed ID12062101
'A Yersinia effector known as YopT and a Pseudomonas avirulence protein known as AvrPphB define a family of 19 proteins involved in bacterial pathogenesis. We show that both YopT and AvrPphB are cysteine proteases, and their proteolytic activities are dependent upon the invariant C/H/D residues conserved in the entire YopT ... More
The role of cadherin, beta-catenin, and AP-1 in retinoid-regulated carcinoma cell differentiation and proliferation.
Authors Shah Salimuddin; Pishvaian Michael J; Easwaran Vijayasurian; Brown Powell H; Byers Stephen W;
JournalJ Biol Chem
PubMed ID12000762
'Vitamin A derivatives (retinoids) are potent regulators of cell proliferation and differentiation. Retinoids inhibit the function of the oncogenic AP-1 and beta-catenin/TCF pathways and also stabilize components of the adherens junction, a tumor suppressor complex. When treated with retinoic acid (RA), the breast cancer cell line, SKBR3, undergoes differentiation and ... More
Expression and localization of the mouse homologue of the yeast V-ATPase 21-kDa Subunit c
Authors Nishi T; Kawasaki-Nishi S; Forgac M;
JournalJ Biol Chem
PubMed ID11441017
We have identified a cDNA encoding the mouse homologue of the yeast V-ATPase 21-kDa subunit c
Plaque production by the polyoma virus.
AuthorsDULBECCO R, FREEMAN G,
JournalVirology
PubMed ID13669362
Describes the original formulation of DMEM (Dulbecco’s Modified Eagle Medium), used for plaque production by the polyoma virus.
Modulation of postendocytic sorting of G protein-coupled receptors.
AuthorsWhistler JL, Enquist J, Marley A, Fong J, Gladher F, Tsuruda P, Murray SR, Von Zastrow M,
JournalScience
PubMed ID12142540
Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding ... More
Interaction between GRIP and Liprin-alpha/SYD2 Is Required for AMPA Receptor Targeting.
Authors Wyszynski Michael; Kim Eunjoon; Dunah Anthone W; Passafaro Maria; Valtschanoff Juli G; Serra-Pagès Carles; Streuli Michel; Weinberg Richard J; Sheng Morgan;
JournalNeuron
PubMed ID11931740
Interaction with the multi-PDZ protein GRIP is required for the synaptic targeting of AMPA receptors, but the underlying mechanism is unknown. We show that GRIP binds to the liprin-alpha/SYD2 family of proteins that interact with LAR receptor protein tyrosine phosphatases (LAR-RPTPs) and that are implicated in presynaptic development. In neurons, ... More
Antiapoptotic activity of the free caspase recruitment domain of procaspase-9: a novel endogenous rescue pathway in cell death.
Authors Stephanou Anastasis; Scarabelli Tiziano M; Knight Richard A; Latchman David S;
JournalJ Biol Chem
PubMed ID11825888
Mitochondrial injury initiates proteolytic processing of procaspase-9 into the large and small subunits, leading to apoptotic cell death. Here we show that the free caspase recruitment domain (CARD) released by procaspase-9 processing activates nuclear factor kappaB expression. A procaspase-9 construct with a point mutation that abrogates the release of the ... More
Activation of Na+-H+ exchange is necessary for RhoA-induced stress fiber formation.
Authors Vexler Z S; Symons M; Barber D L;
JournalJ Biol Chem
PubMed ID8798382
The ubiquitously expressed Na+-H+ exchanger isoform, NHE1, functions in regulating intracellular pH and cell volume. We recently determined that the GTPase Galpha13 stimulates NHE1 activity through a RhoA-dependent mechanism (Hooley, R., Yu, C.-Y., Symons, M., and Barber, D. L. (1996) J. Biol. Chem. 271, 6152-6158). RhoA belongs to the Ras ... More
ACTH-induced nucleocytoplasmic translocation of salt-inducible kinase. Implication in the protein kinase A-activated gene transcription in mouse adrenocortical tumor cells.
AuthorsTakemori H, Katoh Y, Horike N, Doi J, Okamoto M,
JournalJ Biol Chem
PubMed ID12200423
Salt-inducible kinase (SIK), a serine/threonine protein kinase expressed at an early stage of adrenocorticotropic hormone (ACTH) stimulation in Y1 mouse adrenocortical tumor cells, repressed the cAMP-responsive element (CRE)-dependent gene transcription by acting on the basic leucine zipper domain of the CRE-binding protein (Doi, J., Takemori, H., Lin, X.-z., Horike, N., ... More
Retrovirus-derived vectors are overwhelmingly preferred over other methods for ex vivo gene therapy because they provide permanent integration of foreign genes into cellular DNA. In comparison, cationic lipids mediate efficent gene transfer, but expression is transient. When we combined cationic lipids with retrovirus particles we obtained a significant enhancement of ... More
Efficient intracellular assembly of papillomaviral vectors.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient ... More
The novel protein-tyrosine phosphatase PTP20 is a positive regulator of PC12 cell neuronal differentiation.
Authors Aoki N; Yamaguchi-Aoki Y; Ullrich A;
JournalJ Biol Chem
PubMed ID8910608
A novel cytoplasmic protein-tyrosine phosphatase (PTPase) designated PTP20 was isolated from a PC12 cDNA library and shown to positively regulate the differentiation process in PC12 cells. The PTP20 open reading frame of 453 amino acids contains a single tyrosine phosphatase catalytic domain and displays closest homology to members of the ... More
Extracellular glycosaminoglycans modify cellular trafficking of lipoplexes and polyplexes.
Authors Ruponen M; Rönkkö S; Honkakoski P; Pelkonen J; Tammi M; Urtti A;
JournalJ Biol Chem
PubMed ID11390375
It has been shown that extracellular glycosaminoglycans (GAGs) limit the gene transfer by cationic lipids and polymers. The purpose of this study was to clarify how interactions with anionic GAGs (hyaluronic acid and heparan sulfate) modify the cellular uptake and distribution of lipoplexes and polyplexes. Experiments on cellular DNA uptake ... More
G beta gamma subunits mediate mitogen-activated protein kinase activation by the tyrosine kinase insulin-like growth factor 1 receptor.
Authors Luttrell L M; van Biesen T; Hawes B E; Koch W J; Touhara K; Lefkowitz R J;
JournalJ Biol Chem
PubMed ID7622449
The receptors for insulin-like growth factor 1 (IGF1) and insulin are related heterotetrameric proteins which, like the epidermal growth factor (EGF) receptor, possess intrinsic ligand-stimulated tyrosine protein kinase activity. In Rat 1 fibroblasts, stimulation of mitogen-activated protein (MAP) kinase via the IGF1 receptor and the Gi-coupled receptor for lysophosphatidic acid ... More
Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.
Authors Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;
JournalNat Med
PubMed ID12118246
Both enteroviral infection of the heart and mutations in the dystrophin gene can cause cardiomyopathy. Little is known, however, about the interaction between genetic and acquired forms of cardiomyopathy. We previously demonstrated that the enteroviral protease 2A cleaves dystrophin; therefore, we hypothesized that dystrophin deficiency would predispose to enterovirus-induced cardiomyopathy. ... More
Transcriptional regulation of the membrane-associated prostaglandin E2 synthase gene. Essential role of the transcription factor Egr-1.
Membrane-associated prostaglandin (PG) E2 synthase (mPGES) is an inducible terminal enzyme in the biosynthetic pathway for prostaglandin E2, which participates in many biological processes. In this study, we investigated the molecular mechanism controlling the inducible expression of mPGES. The mouse mPGES gene consisted of three exons, and its 5'-proximal promoter ... More
A novel specific role for I kappa B kinase complex-associated protein in cytosolic stress signaling.
We demonstrate here a novel role for the I kappa B kinase complex-associated protein (IKAP) in the regulation of activation of the mammalian stress response via the c-Jun N-terminal kinase (JNK)-signaling pathway. We cloned IKAP as a JNK-associating protein using the Ras recruitment yeast two-hybrid system. IKAP efficiently and specifically ... More
Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading.
Authors Kulkarni Sucheta; Goll Darrel E; Fox Joan E B;
JournalJ Biol Chem
PubMed ID11964413
Integrin-induced cell adhesion results in transmission of signals that induce cytoskeletal reorganizations and resulting changes in cell behavior. The cytoskeletal reorganizations are regulated by transient activation and inactivation of Rho GTPases. Previously, we identified mu-calpain as an enzyme that is activated by signaling across beta1 and beta3 integrins. We showed ... More
Differential association of HLA-B*2705 and B*2709 to ankylosing spondylitis correlates with limited peptide subsets but not with altered cell surface stability.
Authors Ramos Manuel; Paradela Alberto; Vazquez Miriam; Marina Anabel; Vazquez Jesus; Lopez de Castro Jose A;
JournalJ Biol Chem
PubMed ID12042320
In contrast to HLA-B*2705, B*2709 is weakly or not associated to ankylosing spondylitis. Both allotypes differ by a single D116H change. We compared the B*2705- and B*2709-bound peptide repertoires by mass spectrometry to quantify the effect of B*2709 polymorphism on peptide specificity. In addition, shared and differentially bound ligands were ... More
Activation of human monoamine oxidase B gene expression by a protein kinase C MAPK signal transduction pathway involves c-Jun and Egr-1.
Authors Wong Wai K; Ou Xiao-Ming; Chen Kevin; Shih Jean C;
JournalJ Biol Chem
PubMed ID11956220
Monoamine oxidases (MAO) A and B deaminate a number of biogenic amines. Aberrant expression of MAO is implicated in several psychiatric and neurogenerative disorders. In this study, we have shown that phorbol 12-myristate 13-acetate (PMA) increases human MAO B, but not MAO A, gene expression. The sequence between -246 and ... More
Wortmannin-sensitive activation of p70s6k by endogenous and heterologously expressed Gi-coupled receptors.
Authors Wilson M; Burt A R; Milligan G; Anderson N G;
JournalJ Biol Chem
PubMed ID8621477
In order to study the regulation of the ribosomal protein S6 kinase, p70s6k, by G protein-coupled receptors, Rat-1 fibroblasts were stably transfected with two versions of the alpha2 adrenergic receptor. Stimulation of clone 1C cells, which express 3.5 pmol/mg of protein of the human alpha2C10 receptor, with the alpha2 agonist ... More
Krüppel-like zinc fingers bind to nuclear import proteins and are required for efficient nuclear localization of erythroid Krüppel-like factor.
Authors Quadrini Karen J; Bieker James J;
JournalJ Biol Chem
PubMed ID12072445
Erythroid Krüppel-like Factor (EKLF/KLF-1) is an erythroid-specific transcription factor that contains three C(2)H(2) zinc fingers and is required for correct chromatin structure and expression of the beta-globin locus. However, regions within the EKLF protein that serve as signals for its nuclear localization and the proteins that may enable it to ... More
Purification, Cloning, and Characterization of Nek8, a Novel NIMA-related Kinase, and Its Candidate Substrate Bicd2.
Authors Holland Pamela M; Milne Alison; Garka Kirsten; Johnson Richard S; Willis Cynthia; Sims John E; Rauch Charles T; Bird Timothy A; Virca G Duke;
JournalJ Biol Chem
PubMed ID11864968
We describe the isolation, cloning, and characterization of human Nek8, a new mammalian NIMA-related kinase, and its candidate substrate Bicd2. Nek8 was isolated as a beta-casein kinase activity in rabbit lung and has an N-terminal catalytic domain homologous to the Nek family of protein kinases. Nek8 also contains a central ... More
Regulation of enhanced vacuolar H+-ATPase expression in macrophages.
Authors Wang Shui-Ping; Krits Irina; Bai Shuting; Lee Beth S;
JournalJ Biol Chem
PubMed ID11786555
The proton-translocating vacuolar ATPase (V-ATPase) acidifies the endocytic network of eukaryotic cells. Although all eukaryotic cell types require low to moderate levels of V-ATPase, some proton-secreting cells express amplified levels for use in specialized membrane domains. To characterize genetic elements required for this heightened expression, we studied transcription and stability ... More
Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity.
Authors Guan Ennan; Wang Jinhai; Roderiquez Gregory; Norcross Michael A;
JournalJ Biol Chem
PubMed ID12070155
Activated lymphocytes synthesize and secrete substantial amounts of the beta-chemokines macrophage inflammatory protein (MIP)-1 alpha/CCL3 and MIP-1 beta/CCL4, both of which inhibit infection of cells with human immunodeficiency virus type 1 (HIV-1). The native form of MIP-1 beta secreted by activated human peripheral blood lymphocytes (MIP-1 beta(3-69)) lacks the two ... More
Interaction between the nucleocapsid protein and the phosphoprotein of human parainfluenza virus 3. Mapping of the interacting domains using a two-hybrid system.
Authors Zhao H; Banerjee A K;
JournalJ Biol Chem
PubMed ID7759493
A two-hybrid system was used to study interaction in vivo between the nucleocapsid protein (NP) and the phosphoprotein (P) of human parainfluenza virus type 3 (HPIV-3). Two plasmids, one containing the amino terminus of P fused to the DNA-binding domain of the yeast transactivator, GAL4, and the other containing the ... More
Insulin-like growth factor-binding protein 5 (IGFBP-5) interacts with a four and a half LIM protein 2 (FHL2).
Authors Amaar Yousef G; Thompson Garrett R; Linkhart Thomas A; Chen Shin-Tai; Baylink David J; Mohan Subburaman;
JournalJ Biol Chem
PubMed ID11821401
Recent studies using insulin-like growth factor I (IGF-I) knockout mice demonstrate that IGF-binding protein (IGFBP)-5, an important bone formation regulator, itself is a growth factor with cellular effects not dependent on IGFs. Because IGFBP-5 contains a nuclear localization sequence that mediates transport of IGFBP-5 into the nucleus, we propose that ... More
Caspase activation of mammalian sterile 20-like kinase 3 (Mst3). Nuclear translocation and induction of apoptosis.
Authors Huang Chi-Ying F; Wu Yi-Mi; Hsu Chiung-Yueh; Lee Wan-Shu; Lai Ming-Derg; Lu Te-Jung; Huang Chia-Lin; Leu Tzeng-Horng; Shih Hsiu-Ming; Fang Hsin-I; Robinson Dan R; Kung Hsing-Jien; Yuan Chiun-Jye;
JournalJ Biol Chem
PubMed ID12107159
Mammalian Sterile 20-like kinase 3 (Mst3), the physiological functions of which are unknown, is a member of the germinal center kinase-III family. It contains a conserved kinase domain at its NH(2) terminus, whereas there is a regulatory domain at its COOH terminus. In this study we demonstrate that endogenous Mst3 ... More
Trypanosoma cruzi trans-sialidase: a potent and specific survival factor for human Schwann cells by means of phosphatidylinositol 3-kinase/Akt signaling.
Authors Chuenkova M V; Furnari F B; Cavenee W K; Pereira M A;
JournalProc Natl Acad Sci U S A
PubMed ID11481434
Patients infected with Trypanosoma cruzi may remain asymptomatic for decades and show signs of neuroregeneration in the peripheral nervous system (PNS). In the absence of such neuroregeneration, patients may die in part by extensive neuronal destruction in the gastrointestinal tract. Thus, T. cruzi may, despite their invasion of the PNS, ... More
Combinatorial expression of GATA4, Nkx2-5, and serum response factor directs early cardiac gene activity.
Herein, the restricted expression of serum response factors (SRF) closely overlapped with Nkx2-5 and GATA4 transcripts in early chick embryos coinciding with the earliest appearance of cardiac alpha-actin (alphaCA) transcripts and nascent myocardial cells. The combinatorial expression of SRF, a MADS box factor Nkx2-5 (a NK4 homeodomain), and/or GATA4, a ... More
A novel lipoarabinomannan from the equine pathogen Rhodococcus equi. Structure and effect on macrophage cytokine production.
Rhodococcus equi is a major cause of foal morbidity and mortality. We have investigated the presence of lipoglycan in this organism as closely related bacteria, notably Mycobacterium tuberculosis, produce lipoarabinomannans (LAM) that may play multiple roles as virulence determinants. The lipoglycan was structurally characterized by gas chromatography-mass spectrometry following permethylation, ... More
Expression of human fibroblast growth factor 2 mRNA is post-transcriptionally controlled by a unique destabilizing element present in the 3'-untranslated region between alternative polyadenylation sites.
Authors Touriol C; Morillon A; Gensac M C; Prats H; Prats A C;
JournalJ Biol Chem
PubMed ID10409702
Fibroblast growth factor 2 (FGF-2) belongs to a family of 18 genes coding for either mitogenic differentiating factors or oncogenic proteins, the expression of which must be tightly controlled. We looked for regulatory elements in the 5823-nucleotide-long 3'-untranslated region of the FGF-2 mRNA that contains eight potential alternative polyadenylation sites. ... More
Tumor necrosis factor-alpha-induced adipose-related protein (TIARP), a cell-surface protein that is highly induced by tumor necrosis factor-alpha and adipose conversion.
Tumor necrosis factor-alpha (TNF alpha) is involved in the physiological and biological abnormalities found in two opposite metabolic situations: cachexia and obesity. In an attempt to identify novel genes and proteins that could mediate the effects of TNFalpha on adipocyte metabolism and development, we have used a differential display technique ... More
DNA binding and gene activation properties of the Nmp4 nuclear matrix transcription factors.
Authors Torrungruang Kitti; Alvarez Marta; Shah Rita; Onyia Jude E; Rhodes Simon J; Bidwell Joseph P;
JournalJ Biol Chem
PubMed ID11867614
Splice variants of the Nmp4 gene include nuclear matrix transcription factors that regulate the type I collagen alpha1(I) polypeptide chain (COL1A1) promoter and several matrix metalloproteinase (MMP) genes. To date, these are the only Cys(2)His(2) zinc finger proteins known to bind within the minor groove of homopolymeric (dA.dT) DNA. Nmp4 ... More
Cholesterol modulates the membrane binding and intracellular distribution of annexin 6.
Authors de Diego Iñaki; Schwartz Felix; Siegfried Heide; Dauterstedt Paul; Heeren Joerg; Beisiegel Ulrike; Enrich Carlos; Grewal Thomas;
JournalJ Biol Chem
PubMed ID12070178
Annexins are Ca(2+)- and phospholipid-binding proteins that are widely expressed in mammalian tissues and that bind to different cellular membranes. In recent years its role in membrane traffic has emerged as one of its predominant functions, but the regulation of its intracellular distribution still remains unclear. We demonstrated that annexin ... More
Mechanisms of FOXC2- and FOXD1-mediated regulation of the RI alpha subunit of cAMP-dependent protein kinase include release of transcriptional repression and activation by protein kinase B alpha and cAMP.
Leukocyte elastase negatively regulates Stromal cell-derived factor-1 (SDF-1)/CXCR4 binding and functions by amino-terminal processing of SDF-1 and CXCR4.
Activation of CXCR4 by the CXC chemokine stromal cell-derived factor-1 (SDF-1) requires interaction of the amino-terminal domains of both molecules. We report that proteinases released from either mononucleated blood cells or polymorphonuclear neutrophils degranulated by inflammatory stimuli generate an SDF-1 fragment that is deleted from amino-terminal residues Lys(1)-Pro(2)-Val(3), as characterized ... More
Synthesis and biological evaluation in vitro of a selective, high potency peptide agonist of human melanin-concentrating hormone action at human melanin-concentrating hormone receptor 1.
Authors Bednarek Maria A; Tan Carina; Hreniuk Donna L; Palyha Oksana C; MacNeil Douglas J; Van Der Ploeg Lex H Y; Howard Andrew D; Feighner Scott D;
JournalJ Biol Chem
PubMed ID11839762
Human melanin-concentrating hormone (hMCH) is a nonselective natural ligand for the human melanin-concentrating hormone receptors: hMCH-1R and hMCH-2R. Similarly, the smaller peptide encompassing the disulfide ring and Arg(6) of hMCH, Ac-Arg(6)-cyclo(S-S)(Cys(7)-Met(8)-Leu(9)-Gly(10)-Arg(11)-Val(12)-Tyr(13)-Arg(14)-Pro(15)-Cys(16))-NH(2), Ac-hMCH(6-16)-NH(2), binds to and activates equally well both human MCH receptors present in the brain. To separate the physiological ... More
Expression analysis and subcellular distribution of the two G-protein regulators AGS3 and LGN indicate distinct functionality. Localization of LGN to the midbody during cytokinesis.
Authors Blumer Joe B; Chandler L Judson; Lanier Stephen M;
JournalJ Biol Chem
PubMed ID11832491
Activator of G-protein signaling 3 (AGS3) and LGN have a similar domain structure and contain four G-protein regulatory motifs that serve as anchors for the binding of the GDP-bound conformation of specific G-protein alpha subunits. As an initial approach to define further the different functional roles of AGS3 and LGN, ... More
The phosphorylated form of the ORF3 protein of hepatitis E virus interacts with its non-glycosylated form of the major capsid protein, ORF2.
Hepatitis E virus (HEV) is a human RNA virus containing three open reading frames. Of these, ORF1 encodes the viral nonstructural polyprotein; ORF2 encodes the major capsid protein, which exists in a glycosylated and non-glycosylated form; and ORF3 codes for a phosphoprotein of undefined function. Using fluorescence-based colocalization, yeast two-hybrid ... More
Identification of a mitochondrial target of thiazolidinedione insulin sensitizers (mTOT)--relationship to newly identified mitochondrial pyruvate carrier proteins.
AuthorsColca JR, McDonald WG, Cavey GS, Cole SL, Holewa DD, Brightwell-Conrad AS, Wolfe CL, Wheeler JS, Coulter KR, Kilkuskie PM, Gracheva E, Korshunova Y, Trusgnich M, Karr R, Wiley SE, Divakaruni AS, Murphy AN, Vigueira PA, Finck BN, Kletzien RF
Journal
PubMed ID23690925
Thiazolidinedione (TZD) insulin sensitizers have the potential to effectively treat a number of human diseases, however the currently available agents have dose-limiting side effects that are mediated via activation of the transcription factor PPAR?. We have recently shown PPAR?-independent actions of TZD insulin sensitizers, but the molecular target of these ... More
Enhanced activity of variant phospholipase C-delta1 protein (R257H) detected in patients with coronary artery spasm.
BACKGROUND: We recently demonstrated that phospholipase C (PLC)-delta1 activity in cultured skin fibroblasts obtained from patients with coronary spastic angina (CSA) is enhanced. We tested the hypothesis that structural abnormality in PLC-delta1 isoform is a cause of the enhanced activity. METHODS AND RESULTS: Sequence analysis of the cDNA coding for ... More
Glycogen synthase kinase 3beta is activated by cAMP and plays an active role in the regulation of melanogenesis.
In human and mouse, cAMP plays a key role in the control of pigmentation. cAMP, through the activation of protein kinase A, increases the expression of microphthalmia-associated transcription factor (MITF), which in turn stimulates tyrosinase gene expression, to allow melanin synthesis. Beyond this simplified scheme, cAMP inhibits phosphatidylinositol 3-kinase (PI3K), ... More
ANGPTL3 Stimulates Endothelial Cell Adhesion and Migration via Integrin alpha vbeta 3 and Induces Blood Vessel Formation in Vivo.
The angiopoietin family of secreted factors is functionally defined by the C-terminal fibrinogen (FBN)-like domain, which mediates binding to the Tie2 receptor and thereby facilitates a cascade of events ultimately regulating blood vessel formation. By screening expressed sequence tag data bases for homologies to a consensus FBN-like motive, we have ... More
Members of the tristetraprolin family of tandem CCCH zinc finger proteins exhibit CRM1-dependent nucleocytoplasmic shuttling.
Authors Phillips Ruth S; Ramos Silvia B V; Blackshear Perry J;
JournalJ Biol Chem
PubMed ID11796723
Members of the tristetraprolin (TTP) family of CCCH tandem zinc finger proteins can bind directly to certain types of AU-rich elements (AREs) in mRNA. Experiments in TTP-deficient mice have shown that TTP is involved in the physiological destabilization of at least two cytokine mRNAs, those encoding tumor necrosis factor alpha ... More
Human mitochondrial ferritin expressed in HeLa cells incorporates iron and affects cellular iron metabolism.
Mitochondrial ferritin (MtF) is a newly identified ferritin encoded by an intronless gene on chromosome 5q23.1. The mature recombinant MtF has a ferroxidase center and binds iron in vitro similarly to H-ferritin. To explore the structural and functional aspects of MtF, we expressed the following forms in HeLa cells: the ... More
Non-conventional trafficking of the cystic fibrosis transmembrane conductance regulator through the early secretory pathway.
Authors Yoo Jin-San; Moyer Bryan D; Bannykh Sergei; Yoo Hyeon-Mi; Riordan John R; Balch William E;
JournalJ Biol Chem
PubMed ID11799116
The mechanism(s) of cystic fibrosis transmembrane conductance regulator (CFTR) trafficking from the endoplasmic reticulum (ER) through the Golgi apparatus, the step impaired in individuals afflicted with the prevalent CFTR-DeltaF508 mutation leading to cystic fibrosis, is largely unknown. Recent morphological observations suggested that CFTR is largely absent from the Golgi in ... More
JAK-STAT signaling mediates gangliosides-induced inflammatory responses in brain microglial cells.
Authors Kim Ohn Soon; Park Eun Jung; Joe Eun-hye; Jou Ilo;
JournalJ Biol Chem
PubMed ID12191995
Neuronal cell membranes are particularly rich in gangliosides, which play important roles in brain physiology and pathology. Previously, we reported that gangliosides could act as microglial activators and are thus likely to participate in many neuronal diseases. In the present study we provide evidence that JAK-STAT inflammatory signaling mediates gangliosides-stimulated ... More
Influence of second and third cytoplasmic loops on binding, internalization, and coupling of chimeric bombesin/m3 muscarinic receptors.
Authors Tseng M J; Coon S; Stuenkel E; Struk V; Logsdon C D;
JournalJ Biol Chem
PubMed ID7629092
In order to investigate the molecular basis for differences in the characteristics of bombesin (Bn) and m3 muscarinic cholinergic (m3 ACh) receptors, chimeric Bn receptors possessing cytoplasmic domains from the m3 ACh receptor were produced. The receptors were expressed in CHO-K1 cells and binding, structural, and signal transduction characteristics were ... More
Mechanism of 17-beta-estradiol-induced Erk1/2 activation in breast cancer cells. A role for HER2 AND PKC-delta.
Authors Keshamouni Venkateshwar G; Mattingly Raymond R; Reddy Kaladhar B;
JournalJ Biol Chem
PubMed ID11960991
Activation of mitogen-activated protein kinase (Erk/MAPK) is a critical signal transduction event for estrogen (E(2))-mediated cell proliferation. Recent studies from our group and others have shown that persistent activation of Erk plays a major role in cell migration and tumor progression. The signaling mechanism(s) responsible for persistent Erk activation are ... More
A new HIF-1 alpha variant induced by zinc ion suppresses HIF-1-mediated hypoxic responses.
Authors Chun Y S; Choi E; Yeo E J; Lee J H; Kim M S; Park J W;
JournalJ Cell Sci
PubMed ID11739637
The expressions of hypoxia-inducible genes are upregulated by hypoxia-inducible factor 1 (HIF-1), which is a heterodimer of HIF-1alpha and HIF-1beta/ARNT (aryl hydrocarbon receptor nuclear transporter). Under hypoxic conditions, HIF-1alpha becomes stabilized and both HIF-1alpha and ARNT are translocated into the nucleus and codimerized, binding to the HIF-1 consensus sequence and ... More
Apoptotic release of histones from nucleosomes.
Authors Wu Dongcheng; Ingram Alistair; Lahti Jill H; Mazza Brie; Grenet Jose; Kapoor Anil; Liu Lieqi; Kidd Vincent J; Tang Damu;
JournalJ Biol Chem
PubMed ID11812781
Chromatin structure is influenced by histone modification, and this may help direct chromatin behavior to facilitate transcription, DNA replication, and DNA repair. Chromatin condensation and DNA fragmentation are the classic nuclear features but remain poorly characterized. It is highly probable that nucleosomal structure must be altered to allow these features ... More
Molecular basis for pacemaker cells in epithelia.
Authors Leite M Fatima; Hirata Keiji; Pusl Thomas; Burgstahler Angela D; Okazaki Keisuke; Ortega J Miguel; Goes Alfredo M; Prado Marco A M; Spray David C; Nathanson Michael H;
JournalJ Biol Chem
PubMed ID11850419
Intercellular signaling is highly coordinated in excitable tissues such as heart, but the organization of intercellular signaling in epithelia is less clear. We examined Ca(2+) signaling in hepatoma cells expressing the hepatocyte gap junction protein connexin32 (cx32) or the cardiac gap junction protein cx43, plus a fluorescently tagged V(1a) vasopressin ... More
Polyglutamine length-dependent interaction of Hsp40 and Hsp70 family chaperones with truncated N-terminal huntingtin: their role in suppression of aggregation and cellular toxicity.
Authors Jana N R; Tanaka M; Wang G h; Nukina N;
JournalHum Mol Genet
PubMed ID10942430
Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by polyglutamine expansion in the disease protein, huntingtin. In HD patients and transgenic mice, the affected neurons form characteristic ubiquitin-positive nuclear inclusions (NIs). We have established ecdysone-inducible stable mouse Neuro2a cell lines that express truncated N-terminal huntingtin (tNhtt) with different ... More