DMEM,不含葡萄糖
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DMEM,不含葡萄糖
DMEM,不含葡萄糖
DMEM,不含葡萄糖
Gibco™

DMEM,不含葡萄糖

DMEM (Dulbecco's Modified Eagle Medium)是一种广泛使用的基础培养基,可用于支持很多种类的哺乳动物细胞生长。已经在 DMEM 中成功培养出的细胞包括原代成纤维细胞、神经元、胶质细胞了解更多信息
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货号数量
11966025500 mL
货号 11966025
价格(CNY)
397.00
飞享价
Ends: 31-Dec-2025
506.00
共减 109.00 (22%)
Each
添加至购物车
数量:
500 mL
Customize this product
价格(CNY)
397.00
飞享价
Ends: 31-Dec-2025
506.00
共减 109.00 (22%)
Each
添加至购物车
DMEM (Dulbecco's Modified Eagle Medium)是一种广泛使用的基础培养基,可用于支持很多种类的哺乳动物细胞生长。已经在 DMEM 中成功培养出的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVEC、平滑肌细胞,以及 HeLa、293、Cos-7 和 PC-12 等细胞系。针对广泛的细胞培养应用,提供了多种组分的 DMEM 改良培养基。使用培养基选择工具查找适合的配方。

这种 DMEM 的改良方式如下:
包含不含
•L-谷氨酰胺•葡萄糖
•酚红•丙酮酸钠
• HEPES

可提供完整配方

使用 DMEM
DMEM 与其他培养基的不同之处在于其含有相当于初始 Eagle 最低必需培养基 4 倍浓度的氨基酸和维生素。DMEM 最初采用低浓度葡萄糖 (1 g/L) 和丙酮酸钠配制,但通常在含/不含丙酮酸钠的高糖环境下使用。DMEM 不含蛋白质、脂质或生长因子。因此,DMEM 需要补充营养成分,通常需要添加 10% 胎牛血清 (FBS)。DMEM 使用碳酸氢钠缓冲系统 (3.7 g/L),因此需要 5–10% CO2 环境来维持生理 pH 值。

cGMP 生产和质量体系
为确保供应链的稳定,我们同时在位于纽约格兰德岛和英国苏格兰的两家独立工厂生产 DMEM。两家工厂均符合 cGMP 生产要求,并通过 ISO 13485 认证,是在 FDA 登记的医疗器械生产商。
仅供科研使用。不可用于诊断程序。
规格
细胞系HeLa、293、Cos-7 和 PC-12
细胞类型原代成纤维细胞、神经元、神经胶质细胞、HUVEC、平滑肌细胞
最大浓度1 X
生产质量cGMP-compliant under the ISO 13485 standard
产品线Gibco
产品类型DMEM(Dulbecco 改良 Eagle 培养基)
数量500 mL
有效期自生产之日起 12 个月
运输条件室温
分类非动物源性
形式液体
血清水平标准血清
无菌无菌过滤
加有添加剂谷氨酰胺, 酚红
不加添加剂无葡萄糖, 不含 HEPES, 不含丙酮酸钠
Unit SizeEach
内容与储存
储存条件:2°C 至 8°C(避光)
运输条件:环境
有效期:自生产之日起 12 个月

常见问题解答 (FAQ)

My cells will not grow in DMEM, what other type of culture media can be used with the Photoreactive Amino Acids?

Some cells types can be adapted to grow in DMEM before using the DMEM-LM supplemented with the photoreactive amino acids. Currently we do not offer any other leucine- and methionine-depleted culture medium.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (3)

引用和文献
Abstract
Coupling of glucose deprivation with impaired histone H2B monoubiquitination in tumors.
Authors:Urasaki Y, Heath L, Xu CW
Journal:PLoS One
PubMed ID:22615809
Metabolic reprogramming is associated with tumorigenesis. However, glucose metabolism in tumors is poorly understood. Here, we report that glucose levels are significantly lower in bulk tumor specimens than those in normal tissues of the same tissue origins. We show that mono-ubiquitinated histone H2B (uH2B) is a semi-quantitative histone marker for ... More
The role of p53 deacetylation in p21Waf1 regulation by laminar flow.
Authors:Zeng L, Zhang Y, Chien S, Liu X, Shyy JY,
Journal:J Biol Chem
PubMed ID:12716906
Laminar flow arrests vascular endothelial cells at the G0/G1 phase with concurrent increase in p53 and p21Waf1. We investigated the molecular mechanism by which laminar flow activates p53 and p21Waf1 in endothelial cells. The application of a laminar flow (12 dyn/cm2) increased the deacetylation at Lys-320 and Lys-373 of p53 ... More
Glucose uptake mediated by glucose transporter 1 is essential for early tooth morphogenesis and size determination of murine molars.
Authors:Ida-Yonemochi H, Nakatomi M, Harada H, Takata H, Baba O, Ohshima H
Journal:Dev Biol
PubMed ID:22226978
Glucose is an essential source of energy for body metabolism and is transported into cells by glucose transporters (GLUTs). Well-characterized class I GLUT is subdivided into GLUTs1-4, which are selectively expressed depending on tissue glucose requirements. However, there is no available data on the role of GLUTs during tooth development. ... More