Excretory/secretory antigens (ES) from in-vitro cultures of Taenia crassiceps cysticerci, and use of an anti-ES monoclonal antibody for antigen detection in samples of cerebrospinal fluid from patients with neurocysticercosis.
Authors EspÃndola N M; Vaz A J; Pardini A X; Fernandes I;
JournalAnn Trop Med Parasitol
PubMed ID12171617
'Antigens were obtained from cysticerci of the ORF strain of Taenia crassiceps, by culture of cysts in protein-free hybridoma medium (PFHM). Budding of new vesicles was observed after 24-48h. Excretory/secretory (ES) antigens (peptides of <20kDa) were recovered in the medium after culture for 48h. SDS-PAGE analysis of vesicular-fluid (VF) antigens ... More