High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.
Authors Durocher Yves; Perret Sylvie; Kamen Amine;
JournalNucleic Acids Res
PubMed ID11788735
A scalable transfection procedure using polyethylenimine (PEI) is described for the human embryonic kidney 293 cell line grown in suspension. Green fluorescent protein (GFP) and human placental secreted alkaline phosphatase (SEAP) were used as reporter genes to monitor transfection efficiency and productivity. Up to 75% of GFP-positive cells were obtained ... More
Properties and pharmacokinetics of two humanized antibodies specific for L-selectin.
Authors Co M S; Landolfi N F; Nagy J O; Tan J H; Vexler V; Vasquez M; Roark L; Yuan S; Hinton P R; Melrose J; Klingbeil C; Queen C; Berg E L;
JournalImmunotechnology
PubMed ID10231094
BACKGROUND: The participation of L-selectin in leukocyte recruitment during inflammation has suggested the use of L-selectin inhibitors as potential anti-inflammatory therapeutics. Blocking monoclonal antibodies could serve as such therapeutic agents, particularly if humanized to reduce their immunogenicity and improve their serum half-life. OBJECTIVES: For this purpose, two mouse monoclonal antibodies, ... More
Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells.
AuthorsCote J; Garnier A; Massie B; Kamen A;
JournalBiotechnol Bioeng
PubMed ID10099373
This article describes the step-wise approach undertaken to select a serum-free medium (SFM) for the efficient production of a recombinant adenoviral vectors expressing beta-galactosidase (Ad5 CMV-LacZ), in the complementing human embryonic kidney 293S cells. In the first step, a 293S-derived transfectoma, secreting a soluble epidermal growth factor receptor sEGFr (D2-22), ... More