Evaluation of proliferation of neural stem cells in vitro and in vivo.
AuthorsMorte MI, Carreira BP, Machado V, Carmo A, Nunes-Correia I, Carvalho CM, Araújo IM,
JournalCurr Protoc Stem Cell Biol
PubMed ID23404673
This unit describes two basic protocols for the detection of the proliferation of neural stem cells (NSC). The first one addresses cell proliferation in cultures, starting with primary cell cultures isolated from the mouse subventricular zone (SVZ), in which SVZ-derived NSC are kept in culture as neurospheres. By using this ... More