浓度 20 mg RNase A/mL,溶于50 mM Tris-HCl (pH 8.0),10 mM EDTA。
仅供科研使用。不可用于诊断程序。
规格
包装类型瓶装
产品线UltraPure
数量10 mL
运输条件室温
最大浓度20 mg/mL
形式液体
Unit SizeEach
内容与储存
一个 10-ml 瓶
2°C 至 8°C 储存。
常见问题解答 (FAQ)
进行核糖核酸酶保护实验时需要消化总RNA,应使用哪种RNase?
应使用RNAse A或RNase T1消化总RNA。两者的混合物可用于核糖核酸酶保护实验。
I need to digest my total RNA for my ribonuclease protection assay. What RNase should I use?
Total RNA can be digested using RNAse A or RNase T1. A mix of both can be used in ribonuclease protection assays.
引用和文献 (1)
引用和文献
Abstract
Evaluation of proliferation of neural stem cells in vitro and in vivo.
Authors:Morte MI, Carreira BP, Machado V, Carmo A, Nunes-Correia I, Carvalho CM, Araújo IM,
Journal:Curr Protoc Stem Cell Biol
PubMed ID:23404673
This unit describes two basic protocols for the detection of the proliferation of neural stem cells (NSC). The first one addresses cell proliferation in cultures, starting with primary cell cultures isolated from the mouse subventricular zone (SVZ), in which SVZ-derived NSC are kept in culture as neurospheres. By using this ... More