DMEM，粉末，高糖

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Gibco™

DMEM，粉末，高糖

Name: DMEM，粉末，高糖
SKU: 12100046
Price: 870.60 CNY

Dulbecco's Modified Eagle Medium (DMEM) 是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物细胞生长。在 DMEM 中成功培养的细胞包括原代成纤维细胞、神经元了解更多信息

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货号	数量	来源
12100046	10 x 1 L	Primary site of manufacture: Grand Island
12100061	10 L	Primary site of manufacture: Grand Island
52100047 又称 52100-047	50 L	Primary site of manufacture: Inchinnan

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货号 12100046

价格（CNY)

870.60

飞享价

Ends: 31-Dec-2025

1,110.00

共减 239.40 (22%)

Each

添加至购物车

数量:

10 x 1 L

来源:

Primary site of manufacture: Grand Island

Customize this product

价格（CNY)

870.60

飞享价

Ends: 31-Dec-2025

1,110.00

共减 239.40 (22%)

Each

添加至购物车

Dulbecco's Modified Eagle Medium (DMEM) 是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物细胞生长。在 DMEM 中成功培养的细胞包括原代成纤维细胞、神经元、神经胶质细胞、HUVEC、平滑肌细胞，以及 HeLa、293、Cos-7 和 PC-12 等细胞系。我们提供了多种不同的 Gibco™ DMEM 改良培养基，适合广泛的细胞培养应用。使用培养基选择工具查找适合的配方。

这种 DMEM 的改良方式如下：

包含	不含
•高糖	•丙酮酸钠
•L-谷氨酰胺	•HEPES：
•酚红	• 碳酸氢钠

可提供完整配方。

DMEM 与其他培养基的不同之处在于其含有相当于初始 Eagle 最低必需培养基 4 倍浓度的氨基酸和维生素。DMEM 最初采用低浓度葡萄糖 (1 g/L) 和丙酮酸钠配制，但通常在含/不含丙酮酸钠的高糖环境下使用。

cGMP 生产和质量体系
Gibco™ DMEM 在位于英国苏格兰佩斯利的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 注册的医疗器械生产商，且通过 ISO 13485 标准认证。为确保供应链的稳定，我们同时提供由我们格兰德岛工厂生产的相当的 Gibco™ DMEM 产品 (12100-061)。该工厂是在 FDA 登记的医疗器械生产商，且通过 ISO 13485 标准认证。

DMEM 不含蛋白质、脂质或生长因子。因此，DMEM 需要补充营养成分，通常需要添加 10% 胎牛血清 (FBS)。DMEM 使用碳酸氢钠缓冲系统 (3.7 g/L)，因此需要 5-10% CO₂ 环境来维持生理 pH 值。

粉末形式的 Gibco™ 细胞培养基在配制时需要补充碳酸氢钠、调节 pH 值并过滤（有关详细信息，参见方案）。

体外诊断

规格

细胞系HeLa、293、Cos-7 和 PC-12

细胞类型原代成纤维细胞、神经元、神经胶质细胞、HUVEC、平滑肌细胞

生产质量cGMP-compliant under the ISO 13485 standard

产品线Gibco

产品类型DMEM（Dulbecco 改良 Eagle 培养基）

数量10 x 1 L

有效期自生产之日起 36 个月

运输条件室温

来源Primary site of manufacture: Grand Island

分类非动物源性

形式粉末

血清水平标准血清

无菌无菌过滤

加有添加剂高糖, 谷氨酰胺, 酚红

不加添加剂不含 HEPES, 不含丙酮酸钠, 无碳酸氢钠

Unit SizeEach

内容与储存

储存条件：2-8°C
运输条件：环境
有效期：自生产之日起36个月

常见问题解答 (FAQ)

我的粉末培养基一经溶解，其保质期有多久？

以干粉配制的液体培养基与相同配方的在售液体成品一样稳定。通常避光保存于4度冰箱可确保1个月的使用效果。

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (5)

引用和文献

Abstract

Galectin-3 Translocates to the Perinuclear Membranes and Inhibits Cytochrome c Release from the Mitochondria. A ROLE FOR SYNEXIN IN GALECTIN-3 TRANSLOCATION.

Authors: Yu Fei; Finley Russell L Jr; Raz Avraham; Kim Hyeong-Reh Choi;

Journal:J Biol Chem

PubMed ID:11839755

'Galectin-3 is a multifunctional oncogenic protein found in the nucleus and cytoplasm and also the extracellular milieu. Although recent studies demonstrated an anti-apoptotic activity of galectin-3, neither the functional site nor the mechanism of how galectin-3 regulates apoptosis is known. In this study, we examined the subcellular localization of galectin-3 ... More

Functions of Transforming Growth Factor-beta Family Type I Receptors and Smad Proteins in the Hypertrophic Maturation and Osteoblastic Differentiation of Chondrocytes.

Authors: Valcourt Ulrich; Gouttenoire Jerome; Moustakas Aristidis; Herbage Daniel; Mallein-Gerin Frederic;

Journal:J Biol Chem

PubMed ID:12082094

'We investigated the effects of bone morphogenetic protein (BMP)-2, a member of the transforming growth factor-beta superfamily, on the regulation of the chondrocyte phenotype, and we identified signaling molecules involved in this regulation. BMP-2 triggers three concomitant responses in mouse primary chondrocytes and chondrocytic MC615 cells. First, BMP-2 stimulates expression ... More

Vasohibin 2 is transcriptionally activated and promotes angiogenesis in hepatocellular carcinoma.

Authors:Xue X, Gao W, Sun B, Xu Y, Han B, Wang F, Zhang Y, Sun J, Wei J, Lu Z, Zhu Y, Sato Y, Sekido Y, Miao Y, Kondo Y

Journal:Oncogene

PubMed ID:22614011

'Hepatocellular carcinoma (HCC) typically relies on angiogenesis for its malignant behavior, including growth and metastasis. Vasohibin 2 (VASH2) was previously identified as an angiogenic factor, but its role in tumorigenesis is unknown. Using quantitative PCR and western blot analyses, we found that VASH2 is overexpressed in HCC cells and tissues. ... More

Lentiviral vector production, titration, and transduction of primary neurons.

Authors:Ding B, Kilpatrick DL

Journal:

PubMed ID:23681623

'Lentiviral vectors have become very useful tools for transgene delivery. Based on their ability to transduce both dividing and nondividing cells and to produce long-term transgene expression, lentiviruses have found numerous applications in the biomedical sciences, including developmental neuroscience. This protocol describes how to prepare lentiviral vectors by calcium phosphate ... More

Evidence that the transmembrane biogenesis of aquaporin 1 is cotranslational in intact mammalian cells.

Authors: Dohke Yoko; Turner R James;

Journal:J Biol Chem

PubMed ID:11884383

Most polytopic membrane proteins are believed to integrate into the membrane of the endoplasmic reticulum (ER) cotranslationally. However, recent studies with Xenopus oocytes and dog pancreatic microsomes have suggested that this is not the case for human aquaporin 1 (AQP1). These experiments indicate that membrane-spanning segments (MSSs) 2 and 4 ... More