DMEM，粉末，高糖
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DMEM，粉末，高糖
引用和文献 (7)
Gibco™

DMEM，粉末，高糖

Dulbecco's Modified Eagle Medium (DMEM) 是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物细胞生长。在 DMEM 中成功培养的细胞包括原代成纤维细胞、神经元了解更多信息
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货号数量来源
1210006110 LPrimary site of manufacture: Grand Island
1210004610 x 1 LPrimary site of manufacture: Grand Island
52100047
又称 52100-047
50 LPrimary site of manufacture: Inchinnan
货号 12100061
价格（CNY)
425.80
飞享价
Ends: 31-Dec-2025
543.00
共减 117.20 (22%)
Each
添加至购物车
数量:
10 L
来源:
Primary site of manufacture: Grand Island
Customize this product
价格（CNY)
425.80
飞享价
Ends: 31-Dec-2025
543.00
共减 117.20 (22%)
Each
添加至购物车
Dulbecco's Modified Eagle Medium (DMEM) 是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物细胞生长。在 DMEM 中成功培养的细胞包括原代成纤维细胞、神经元、神经胶质细胞、HUVEC、平滑肌细胞，以及 HeLa、293、Cos-7 和 PC-12 等细胞系。我们提供了多种不同的 Gibco™ DMEM 改良培养基，适合广泛的细胞培养应用。使用培养基选择工具查找适合的配方。

这种 DMEM 的改良方式如下：

包含不含
•高糖•丙酮酸钠
•L-谷氨酰胺•HEPES：
•酚红• 碳酸氢钠

可提供完整配方。

DMEM 与其他培养基的不同之处在于其含有相当于初始 Eagle 最低必需培养基 4 倍浓度的氨基酸和维生素。DMEM 最初采用低浓度葡萄糖 (1 g/L) 和丙酮酸钠配制，但通常在含/不含丙酮酸钠的高糖环境下使用。

cGMP 生产和质量体系
Gibco™ DMEM 在位于英国苏格兰佩斯利的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 注册的医疗器械生产商，且通过 ISO 13485 标准认证。为确保供应链的稳定，我们同时提供由我们格兰德岛工厂生产的相当的 Gibco™ DMEM 产品 (12100-061)。该工厂是在 FDA 登记的医疗器械生产商，且通过 ISO 13485 标准认证。

DMEM 不含蛋白质、脂质或生长因子。因此，DMEM 需要补充营养成分，通常需要添加 10% 胎牛血清 (FBS)。DMEM 使用碳酸氢钠缓冲系统 (3.7 g/L)，因此需要 5-10% CO2 环境来维持生理 pH 值。

粉末形式的 Gibco™ 细胞培养基在配制时需要补充碳酸氢钠、调节 pH 值并过滤（有关详细信息，参见方案）。
体外诊断
规格
细胞系HeLa、293、Cos-7 和 PC-12
细胞类型原代成纤维细胞、神经元、神经胶质细胞、HUVEC、平滑肌细胞
葡萄糖浓度4500 mg/L
生产质量cGMP-compliant under the ISO 13485 standard
产品线Gibco
产品类型DMEM（Dulbecco 改良 Eagle 培养基）
数量10 L
有效期自生产之日起 36 个月
运输条件室温
来源Primary site of manufacture: Grand Island
分类非动物源性
形式粉末
血清水平标准血清
无菌无菌过滤
加有添加剂高糖, 谷氨酰胺, 酚红
不加添加剂不含 HEPES, 不含丙酮酸钠, 无碳酸氢钠
Unit SizeEach
内容与储存
储存条件：2-8°C
运输条件：环境
有效期：自生产之日起 36 个月

常见问题解答 (FAQ)

我的粉末培养基一经溶解，其保质期有多久？

以干粉配制的液体培养基与相同配方的在售液体成品一样稳定。通常避光保存于4度冰箱可确保1个月的使用效果。

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (7)

引用和文献
Abstract
Galectin-3 Translocates to the Perinuclear Membranes and Inhibits Cytochrome c Release from the Mitochondria. A ROLE FOR SYNEXIN IN GALECTIN-3 TRANSLOCATION.
Authors: Yu Fei; Finley Russell L Jr; Raz Avraham; Kim Hyeong-Reh Choi;
Journal:J Biol Chem
PubMed ID:11839755
'Galectin-3 is a multifunctional oncogenic protein found in the nucleus and cytoplasm and also the extracellular milieu. Although recent studies demonstrated an anti-apoptotic activity of galectin-3, neither the functional site nor the mechanism of how galectin-3 regulates apoptosis is known. In this study, we examined the subcellular localization of galectin-3 ... More
Cloning and characterization of freac-9 (FKHL17), a novel kidney-expressed human forkhead gene that maps to chromosome 1p32-p34.
Authors: Ernstsson S; Betz R; Lagercrantz S; Larsson C; Ericksson S; Cederberg A; Carlsson P; Enerbäck S;
Journal:Genomics
PubMed ID:9403061
'We describe the cloning of a near full-length cDNA of 4258 nucleotides encoding freac-9 (HGMW-approved symbol FKHL17), a novel human forkhead gene. The 5'' untranslated region is unusual since it is very long, 2127 nucleotides, and contains 15 upstream AUG codons. Hybridization to a panel consisting of RNA derived from ... More
Low intracellular zinc impairs the translocation of activated NF-kappa B to the nuclei in human neuroblastoma IMR-32 cells.
Authors: Mackenzie Gerardo G; Zago M Paola; Keen Carl L; Oteiza Patricia I;
Journal:J Biol Chem
PubMed ID:12089148
'In the current work, we studied how variations in extracellular zinc concentrations modulate different steps involved in nuclear factor kappaB (NF-kappaB) activation in human neuroblastoma IMR-32 cells. Cells were incubated in media containing varying concentrations of zinc (1.5, 5, 15, and 50 microm). Within 3 h, the intracellular zinc content ... More
Tissue-specific regulation of the type X collagen gene. Analyses by in vivo footprinting and transfection with a proximal promoter region.
Authors: Long F; Linsenmayer T F;
Journal:J Biol Chem
PubMed ID:8537401
During endochondral bone formation, hypertrophic chondrocytes initiate synthesis of type X collagen. Previous studies have shown that regulation of this molecule is at the level of transcription. To further explore this regulation, we have studied a segment of the type X collagen gene extending from 562 base pairs (bp) upstream ... More
Targeted disruption of Np95 gene renders murine embryonic stem cells hypersensitive to DNA damaging agents and DNA replication blocks.
Authors: Muto Masahiro; Kanari Yasuyoshi; Kubo Eiko; Takabe Tamami; Kurihara Takayuki; Fujimori Akira; Tatsumi Kouichi;
Journal:J Biol Chem
PubMed ID:12084726
NP95, which contains a ubiquitin-like domain, a cyclin A/E-Cdk2 phosphorylation site, a retinoblastoma (Rb) binding motif, and a ring finger domain, has been shown to be colocalized as foci with proliferating cell nuclear antigen in early and mid-S phase nuclei. We established Np95 nulligous embryonic stem cells by replacing the ... More
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