Comparison of commercial RNA extraction kits for preparation of DNA-free total RNA from Salmonella cells.
AuthorsRump LV, Asamoah B, Gonzalez-Escalona N,
JournalBMC Res Notes
PubMed ID20663210
'The isolation of DNA-free RNA is a crucial step in the reverse transcription PCR (RT-PCR). Every RNA extraction procedure results in RNA samples contaminated with genomic DNA, which can cause false-positive outcomes in highly sensitive applications, including a recently developed quantitative real-time PCR (RT-qPCR) assay that targets invA mRNA for ... More