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Invitrogen™
TRIzol™Plus RNA 纯化试剂盒
The TRIzol™ Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a了解更多信息
| 货号 | 数量 |
|---|---|
| 12183555CN | 50 次制备 |
货号 12183555CN
价格（CNY)
3,866.00
Each
数量:
50 次制备
价格（CNY)
3,866.00
Each
The TRIzol™ Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol™ Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink™ RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.
Features of this kit include:
• Combined TRIzol™-based lysis and PureLink™ spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol
Two products in one
The TRIzol™ Plus RNA Purification System couples the lysis capabilities of TRIzol™ Reagent with the 1000 μg column-;binding capacity of the PureLink™ RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol™ Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol™ Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol™ Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.
Simple lysis to column purification protocol
TRIzol™ Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol™ Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink™ RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
仅供科研使用。不可用于诊断程序。
规格
洗脱体积30 至 300 μL
最终产品类型总 RNA、转录组 RNA、micro RNA
适用于（应用）RT-PCR、qPCR、cDNA 文库构建、NGS、微阵列分析、印迹杂交、Northern 印迹、体外翻译、核酸酶保护测定、核酸标记
高通量能力不兼容高通量应用（手动）
纯化时间1 hr.
数量50 次制备
规格小型
运输条件室温
原始材料量多达 5 x 10^8 个细胞，多达 200 mg 组织
产量1000 μg
分离技术硅胶离心柱
样品类型细菌
Unit SizeEach
内容与储存
试剂盒组成部分：
• TRIzol™ 试剂 (100 mL)
• 裂解缓冲液 (125 mL)
• 洗涤缓冲液 I (50 mL)
• 洗涤缓冲液 II (15 mL)
• 无 RNase 水 (15.5 mL)
• 带收集管的离心柱（各 50 个）
• 收集管（各 50 个）
• 回收管（各 50 个）
所有组分均储存在室温下。提供足够进行 50 次 RNA 分离的试剂。
• TRIzol™ 试剂 (100 mL)
• 裂解缓冲液 (125 mL)
• 洗涤缓冲液 I (50 mL)
• 洗涤缓冲液 II (15 mL)
• 无 RNase 水 (15.5 mL)
• 带收集管的离心柱（各 50 个）
• 收集管（各 50 个）
• 回收管（各 50 个）
所有组分均储存在室温下。提供足够进行 50 次 RNA 分离的试剂。