Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of t(12;13)(p13;q12) acute myeloid leukemia.
AuthorsRawat VP, Cusan M, Deshpande A, Hiddemann W, Quintanilla-Martinez L, Humphries RK, Bohlander SK, Feuring-Buske M, Buske C,
JournalProc Natl Acad Sci U S A
PubMed ID14718672
Creation of fusion genes by balanced chromosomal translocations is one of the hallmarks of acute myeloid leukemia (AML) and is considered one of the key leukemogenic events in this disease. In t(12;13)(p13;q12) AML, ectopic expression of the homeobox gene CDX2 was detected in addition to expression of the ETV6-CDX2 fusion ... More
MICAL, a novel CasL interacting molecule, associates with vimentin.
'CasL/HEF1 belongs to the p130(Cas) family. It is tyrosine-phosphorylated following beta(1) integrin and/or T cell receptor stimulation and is thus considered to be important for immunological reactions. CasL has several structural motifs such as an SH3 domain and a substrate domain and interacts with many molecules through these motifs. To ... More
Analysis of gene expression profiles in normal and neoplastic ovarian tissue samples identifies candidate molecular markers of epithelial ovarian cancer.
' Epithelial ovarian cancer is the leading cause of death from gynecologic cancer, in part because of the lack of effective early detection methods. Although alterations of several genes, such as c-erb-B2, c-myc, and p53, ... More
Divergent regulation of the growth-promoting gene IEX-1 by the p53 tumor suppressor and Sp1.
Authors Im Hee-Jeong; Pittelkow Mark R; Kumar Rajiv;
JournalJ Biol Chem
PubMed ID11844788
'IEX-1, a recently discovered early response gene, regulates cell growth and apoptosis. IEX-1 gene expression is regulated by a variety of factors such as x-irradiation, ultraviolet radiation, steroids, growth factors, and inflammatory stimuli. By systematic examination of the IEX-1 promoter, we show that IEX-1 gene expression is controlled by multiple ... More
Molecular mechanism of the induction of metalloproteinases 1 and 3 in human fibroblasts by basic calcium phosphate crystals. Role of calcium-dependent protein kinase C alpha.
Authors Reuben Paul M; Brogley Michele A; Sun Yubo; Cheung Herman S;
JournalJ Biol Chem
PubMed ID11836255
'Synovial fluid basic calcium phosphate (BCP) crystals are common in osteoarthritis and are often associated with destructive arthropathies involving cartilage degeneration. These crystals are mitogenic and induce oncogene expression and matrix metalloproteinase (MMP) synthesis and secretion in human fibroblasts. To date, BCP crystal-elicited signal transduction pathways have not been completely ... More
Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channels.
Authors Takimoto Koichi; Yang Eun-Kyoung; Conforti Laura;
JournalJ Biol Chem
PubMed ID12006572
'The Ca(2+)-binding proteins KChIP1-4 (KChIP3 is also known as DREAM and calsenilin) act as auxiliary subunits for voltage-gated K(+) channels in the Kv4 family. Here we identify three splicing isoforms of rat KChIP2 with variable N-terminal peptides. The two longer isoforms, which contain the 32-amino acid peptide, produce larger increases ... More
ATP stimulates signal recognition particle (SRP)/FtsY-supported protein integration in chloroplasts.
Authors Yuan Jianguo; Kight Alicia; Goforth Robyn L; Moore Misty; Peterson Eric C; Sakon Joshua; Henry Ralph;
JournalJ Biol Chem
PubMed ID12105232
'The signal recognition particle (SRP) and its receptor (FtsY in prokaryotes) are essential for cotranslational protein targeting to the endoplasmic reticulum in eukaryotes and the cytoplasmic membrane in prokaryotes. An SRP/FtsY-like protein targeting/integration pathway in chloroplasts mediates the posttranslational integration of the light-harvesting chlorophyll a/b-binding protein (LHCP) into thylakoid membranes. ... More
Initiation of tRNA maturation by RNase E is essential for cell viability in E. coli.
Authors Ow Maria C; Kushner Sidney R;
JournalGenes Dev
PubMed ID12000793
'RNase E, an essential endoribonuclease in Escherichia coli, is involved in 9S rRNA processing, the degradation of many mRNAs, and the processing of the M1 RNA subunit of RNase P. However, the reason that RNase E is required for cell viability is still not fully understood. In fact, recent experiments ... More
Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase.
Authors Fehlner-Gardiner Christine; Roshick Christine; Carlson John H; Hughes Scott; Belland Robert J; Caldwell Harlan D; McClarty Grant;
JournalJ Biol Chem
PubMed ID12011099
Here we report the cloning and sequencing of a region of the chlamydiae chromosome termed the
Association of calnexin with mutant peripheral myelin protein-22 ex vivo: a basis for
Authors Dickson K M; Bergeron J J M; Shames I; Colby J; Nguyen D T; Chevet E; Thomas D Y; Snipes G J;
JournalProc Natl Acad Sci U S A
PubMed ID12119418
Schwann cell-derived peripheral myelin protein-22 (PMP-22) when mutated or overexpressed causes heritable neuropathies with a previously unexplained
FCP1 phosphorylation by casein kinase 2 enhances binding to TFIIF and RNA polymerase II carboxyl-terminal domain phosphatase activity.
AuthorsPalancade B, Dubois MF, Bensaude O,
JournalJ Biol Chem
PubMed ID12138108
Dephosphorylation of RNA polymerase II carboxyl-terminal domain (CTD) is required to resume sequential transcription cycles. FCP1 (TFIIF-dependent CTD phosphatase 1) is the only known phosphatase targeting RNAP II CTD. Here we show that in Xenopus laevis cells, xFCP1 is a phosphoprotein. On the basis of biochemical fractionation and drug sensitivity, ... More
Involvement of a bifunctional fatty-acyl desaturase in the biosynthesis of the silkmoth, Bombyx mori, sex pheromone.
The straight-chain C(10) to C(18) unsaturated aliphatic compounds containing an oxygenated functional group (aldehyde, alcohol, or acetate ester) derived from saturated C(16) or C(18) fatty acids are a major class of sex pheromone components produced by female moths. In the biosynthesis of these pheromone components, various combinations of limited chain-shortening ... More
A novel 43-kDa protein as a negative regulatory component of phenoloxidase-induced melanin synthesis.
AuthorsZhao M, Söderhäll I, Park JW, Ma YG, Osaki T, Ha NC, Wu CF, Söderhäll K, Lee BL,
JournalJ Biol Chem
PubMed ID15857824
The melanization reaction induced by activated phenoloxidase in arthropods is important in the multiple host defense innate immune reactions, leading to the sequestration and killing of invading microorganisms. This reaction ought to be tightly controlled because excessive formation of quinones and systemic hypermelanization are deleterious to the hosts, suggesting that ... More
Functional Involvement of the Brahma/SWI2-related Gene 1 Protein in Cytochrome P4501A1 Transcription Mediated by the Aryl Hydrocarbon Receptor Complex.
Authors Wang Song; Hankinson Oliver;
JournalJ Biol Chem
PubMed ID11805098
Chromatin remodeling is a key step in overcoming the nucleosomal repression of active transcription in eukaryotes. The mammalian SWI/SNF ATP-dependent chromatin-remodeling complexes contain multiple subunits. The ATPase activities in these complexes are attributable to either BRG-1 or the related Brahma protein. The aryl hydrocarbon receptor (AHR), after binding xenobiotic ligands ... More