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Citations & References (2)
Invitrogen™
Gateway™ pcDNA™-DEST40 Vector
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway™ pcDNA™-DEST40 vectorRead more
| Catalog Number | Quantity |
|---|---|
| 12274015 also known as 12274-015 | 6 μg |
Catalog number 12274015
also known as 12274-015
Price (CNY)
8,695.86
Online Exclusive
Ends: 31-Dec-2025
10,383.00Save 1,687.14 (16%)
Each
Quantity:
6 μg
Price (CNY)
8,695.86
Online Exclusive
Ends: 31-Dec-2025
10,383.00Save 1,687.14 (16%)
Each
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway™ pcDNA™-DEST40 vector offers the following key features:
•C-terminal V5-6x His tag for easy detection and rapid purification
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
•C-terminal V5-6x His tag for easy detection and rapid purification
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
For Use With (Application)Constitutive Expression
Product TypeGateway System Destination Expression Vector
Quantity6 μg
Selection Agent (Eukaryotic)Geneticin™ (G-418)
VectorpDEST
Cloning MethodGateway
Product LineGateway, pDEST
PromoterCMV
Protein TagHis Tag (6x), V5 Epitope Tag
Unit SizeEach
Contents & Storage
All destination vectors are provided lyophilized and supercoiled.
Frequently asked questions (FAQs)
Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?
Do you have a recommended single-step protocol for BP/LR recombination?
How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?
Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?
Do you offer Gateway vectors for expression in plants?
Citations & References (2)
Citations & References
Abstract
Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance.
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16611727
Mastitis, a mammary gland inflammation in response to bacterial infection, is a major problem in the dairy industry. We found that cows susceptible to mastitis have a three-base insertion in a glycine-coding stretch of the gene for forebrain embryonic zinc finger-like (FEZL), a transcription factor with a role in neuronal
Calpain 3 is activated through autolysis within the active site and lyses sarcomeric and sarcolemmal components.
Journal:Mol Cell Biol
PubMed ID:14645524
Calpain 3 (Capn3) is known as the skeletal muscle-specific member of the calpains, a family of intracellular nonlysosomal cysteine proteases. This enigmatic protease has many unique features among the calpain family and, importantly, mutations in Capn3 have been shown to be responsible for limb girdle muscular dystrophy type 2A. Here