PureLink™ 病毒 RNA/DNA 小提试剂盒
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PureLink™ 病毒 RNA/DNA 小提试剂盒
Invitrogen™

PureLink™ 病毒 RNA/DNA 小提试剂盒

PureLink 病毒 RNA/DNA 小提试剂盒提供了一种快速高效的方法,可同时从新鲜或冷冻的无细胞生物液体(血浆、血清、脑脊液)和细胞培养物上清液中纯化病毒 RNA/DNA。该试剂盒经过专门设计,使用可实现灵敏下游分析的较低洗脱体积就能在45分钟内从多种了解更多信息
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货号数量
1228005050 次制备
货号 12280050
价格(CNY)
1,726.00
飞享价
Ends: 31-Dec-2025
2,297.00
共减 571.00 (25%)
Each
添加至购物车
数量:
50 次制备
价格(CNY)
1,726.00
飞享价
Ends: 31-Dec-2025
2,297.00
共减 571.00 (25%)
Each
添加至购物车
PureLink 病毒 RNA/DNA 小提试剂盒提供了一种快速高效的方法,可同时从新鲜或冷冻的无细胞生物液体(血浆、血清、脑脊液)和细胞培养物上清液中纯化病毒 RNA/DNA。该试剂盒经过专门设计,使用可实现灵敏下游分析的较低洗脱体积就能在45分钟内从多种 RNA 和 DNA 病毒中分离出高质量病毒核酸。纯化的病毒 RNA/DNA 不含蛋白和核酸酶,适用于可实现病毒检测和基因分型的下游应用。

PureLink 病毒小提试剂盒已被用于从正痘病毒(包括牛痘病毒、天花病毒、猴痘病毒、痘苗病毒和 HERV)中纯化病毒 dsDNA。

PureLink 病毒 RNA/DNA 小提试剂盒具有以下优势:
• 使用基于离心柱的离心方法快速、高效地纯化高质量病毒核酸,无样品交叉污染
• 经专门设计,可在45分钟内从 ≤500 µL 无细胞样品中纯化病毒 RNA 和 DNA
• 能够在洗脱体积较低 (10–50 µL) 的条件下洗脱病毒核酸,以便进行灵敏的下游分析

仅供科研使用。不可用于诊断程序。
规格
色谱柱类型离心柱
洗脱体积10 to 50 μL
最终产品类型病毒 DNA⁄RNA
适用于(应用)实时荧光定量 PCR (qPCR)、逆转录酶 PCR (RT-PCR)、Northern 印迹
高通量能力不兼容高通量应用(手动)
数量50 次制备
样品类型细胞, 脑脊液, 液体样品(例如血清), 血浆, 血清, Virus, Nasopharyngeal Viral Transfer Medium (NPVTM)
规格小型
运输条件室温
原始材料量≤500 μL
测试时间45分钟
产量5 μg (Binding capacity)
分离技术离心柱
Unit SizeEach
内容与储存
PureLink™ 病毒 RNA/DNA 小提试剂盒包含足够进行 50 次反应的试剂。

试剂盒含有:
•32 mL 病毒裂解缓冲液 (L22)
•15 mL 5X 洗涤缓冲液 (W5)
•2 × 1.6 mL 蛋白酶 K(20 mg/mL,溶于储存缓冲液)
•310 µg 冻干载体 RNA
•15.5 mL 无菌无 RNAase 水
•50 病毒离心柱与收集管
•2 × 50 洗涤管 (2.0 mL)
•50 回收管 (1.5 mL)

在室温下储存除载体 RNA 外的所有组分。载体 RNA 应在 −20°C 下储存。

常见问题解答 (FAQ)

Are additional wash tubes available for the PureLink Viral RNA/DNA kits if I do not want to use the wash columns twice?

Yes, the Cat. No. for 100 wash tubes is 12282-100.

What is the stability of the Proteinase K in the PureLink Viral RNA/DNA kits?

The Proteinase K solution is stable for 1 year when stored at room temperature. For long-term storage (>1 year) or if room temperature is >25 degrees C, store the Proteinase K solution at 4 degrees C.

What is the difference between serum and plasma?

Plasma is the liquid portion of the blood that is separated from the blood cells by centrifugation. One of the characteristics of plasma is that it clots easily, which is important for hemophiliacs needing a transfusion but is a nuisance in most other applications. By agitating the plasma, one can precipitate the clotting factors as a large clot, and the leftover fluid is called serum. In other words, serum plus clotting factors is plasma, and clotted plasma yields serum.

What is the stability of the carrier RNA in the PureLink Viral RNA/DNA kit, both lyophilized and in solution?

The tRNA is stable for over one year. There was no significant difference in qRT-PCR results with tRNA stored for 10 days at ‘20 degrees C or 42 degrees C. The lyophilized tRNA is stable at ‘20 degrees C, room temperature, 37 degrees C, and 45 degrees C for up to 1 month.

Do I need to use the carrier RNA in the PureLink Viral RNA/DNA kit?

The carrier RNA is tRNA and it may not be necessary. We have tested without the tRNA and obtained excellent results. The tRNA provides a substrate for RNases in the sample to protect the viral RNA until the virus is lysed. You may want to scale down the amount of carrier RNA or leave it out. However, you should validate it in your application to make sure you get the same results as when you include it.