Gateway™ pYES-DEST52 Vector
Product Image
Invitrogen™

Gateway™ pYES-DEST52 Vector

The pYES2-DEST52 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombinationRead more
Have Questions?
Catalog NumberQuantity
122860196 μg
Catalog number 12286019
Price (CNY)
6,434.79
Online Exclusive
Ends: 31-Dec-2025
7,685.00
Save 1,250.21 (16%)
Each
Add to cart
Quantity:
6 μg
Price (CNY)
6,434.79
Online Exclusive
Ends: 31-Dec-2025
7,685.00
Save 1,250.21 (16%)
Each
Add to cart
The pYES2-DEST52 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent expression in Saccharomyces cerevisiae. As part of the YES™ Vector Collection, pYES2-DEST52 carries the promoter and enhancer sequences from the GAL1 gene for regulated expression in S.cerevisiae.
pYES2-DEST52 features:

• 2μ origin of replication for high-copy maintenance
gene for auxotrophic selection on economical minimal medium
• C-terminal V5 epitope and polyhistidine(6xHis) tags for efficient detection and purification
tR sites for efficient recombination with any attL-flanked Gateway™ entry vector
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialAmpicillin (AmpR)
Product TypeGateway System Destination Expression Vector
Quantity6 μg
VectorpYES, pDEST
Cloning MethodGateway
Product LineGateway™
PromoterGAL1
Protein TagHis Tag (6x), V5 Epitope Tag
Unit SizeEach
Contents & Storage
The pYES2-DEST52 Gateway™ destination vector is provided supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pYES-DEST52 Vector FAQs