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引用和文献 (10)
Invitrogen™
Gateway™ pYES-DEST52 载体
pYES2-DEST52 Gateway™ 目的载体旨在使用 λ 噬菌体位点特异性重组对 Gateway™ 入门克隆进行快速克隆以及随后在酿酒酵母中进行表达。作为 YES™ 载体系列的一部分，pYES2-DEST52了解更多信息
| 货号 | 数量 |
|---|---|
| 12286019 | 6 μg |
货号 12286019
价格（CNY)
6,434.79
飞享价
Ends: 31-Dec-2025
7,685.00共减 1,250.21 (16%)
Each
数量:
6 μg
价格（CNY)
6,434.79
飞享价
Ends: 31-Dec-2025
7,685.00共减 1,250.21 (16%)
Each
pYES2-DEST52 Gateway™ 目的载体旨在使用 λ 噬菌体位点特异性重组对 Gateway™ 入门克隆进行快速克隆以及随后在酿酒酵母中进行表达。作为 YES™ 载体系列的一部分，pYES2-DEST52 携带来自 GAL1 基因的启动子和增强子序列，用于在酿酒酵母中进行调节性表达。
pYES2-DEST52 具有以下特点：
•2µ origin 复制用于高拷贝维持
采用经济的基本培养基对基因进行营养缺陷型筛选
• C 端 V5 抗原决定簇和多组氨酸 (6xHis) 标记实现高效检测和纯化
tR 位点使用任何 attL 侧区 Gateway™ 入门载体进行高效重组
pYES2-DEST52 具有以下特点：
•2µ origin 复制用于高拷贝维持
采用经济的基本培养基对基因进行营养缺陷型筛选
• C 端 V5 抗原决定簇和多组氨酸 (6xHis) 标记实现高效检测和纯化
tR 位点使用任何 attL 侧区 Gateway™ 入门载体进行高效重组
仅供科研使用。不可用于诊断程序。
规格
耐抗生素细菌氨苄青霉素 (AmpR)
产品类型Gateway 系统目的表达载体
数量6 μg
载体pYES、pDEST
克隆方法Gateway™
产品线Gateway
促进剂GAL1
蛋白标记His 标签 (6x)、V5 抗原决定簇标签, V5 Epitope Tag
Unit SizeEach
内容与储存
pYES2-DEST52 Gateway™ 目的载体以超螺旋化冻干形式提供。在 -20°C 下储存。妥善储存时，载体可保证稳定储存 6 个月。
常见问题解答 (FAQ)
可以使用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶LR Clonase II酶进行BP/LR Clonase反应的一步法实验方案吗？
有推荐的一步式BP/LR重组实验方案吗？
如果丢失了入门克隆，如何将目的基因从一个Gateway兼容的表达克隆转移到一个新的目的载体？
我可以单独购买5X LR Clonase缓冲液或5X BP Clonase缓冲液吗？
是否提供用于在植物内表达的Gateway载体吗？
引用和文献 (10)
引用和文献
Abstract
Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
Journal:J Biol Chem
PubMed ID:16162496
'A large number of post-transcriptional base modifications in transfer RNAs have been described (Sprinzl, M., Horn, C., Brown, M., Ioudovitch, A., and Steinberg, S. (1998) Nucleic Acids Res. 26, 148-153). These modifications enhance and expand tRNA function to increase cell viability. The intermediates and genes essential for base modifications in
Participation of an Endomembrane Cation/H+ Exchanger AtCHX20 in Osmoregulation of Guard Cells.
Journal:Plant Physiol
PubMed ID:17337534
'Guard cell movement is induced by environmental and hormonal signals that cause changes in turgor through changes in uptake or release of solutes and water. Several transporters mediating these fluxes at the plasma membrane have been characterized, however less is known about transport at endomembranes. CHX20, a member of a
Identification of a receptor-independent activator of G protein signaling (AGS8) in ischemic heart and its interaction with Gbetagamma.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16407149
'As part of a broader effort to identify postreceptor signal regulators involved in specific diseases or organ adaptation, we used an expression cloning system in Saccharomyces cerevisiae to screen cDNA libraries from rat ischemic myocardium, human heart, and a prostate leiomyosarcoma for entities that activated G protein signaling in the
Isolation and characterization of cDNAs encoding an enzyme with glucosyltransferase activity for cyclo-DOPA from four o'clocks and feather cockscombs.
Journal:Plant Cell Physiol
PubMed ID:15695438
cDNAs encoding an enzyme with UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity were isolated from four o'clocks and feather cockscombs. Phylogenetic analysis of the amino acid sequences deduced from the cDNAs show that they represent a single subclade distinct from those of other phenylpropanoid and flavonoid glucosyltransferases. Changes in the amount of transcripts of
Molecular and Biochemical Characterization of a Novel Intracellular Invertase from Aspergillus niger with Transfructosylating Activity.
Journal:Eukaryot Cell
PubMed ID:17293485
A novel sub-family of putative intracellular invertase enzymes (glycoside hydrolase family 32) has previously been identified in fungal genomes. Here we report phylogenetic, molecular and biochemical characteristics of SucB, one of two novel intracellular invertases identified in Aspergillus niger. The sucB gene was expressed in Escherichia coli and an invertase