Dual-tagging system for the affinity purification of mammalian protein complexes.
AuthorsGiannone RJ, McDonald WH, Hurst GB, Huang Y, Wu J, Liu Y, Wang Y,
JournalBiotechniques
PubMed ID17907572
'Although affinity purification coupled with mass spectrometry (MS) provides a powerful tool to study protein-protein interactions, this strategy has encountered numerous difficulties when adapted to mammalian cells. Here we describe a Gateway-compatible dual-tag affinity purification system that integrates regulatable expression, tetracysteine motifs, and various combinations ofaffinity tags to facilitate the ... More