One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells - FAQs

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2 个常见问题解答

你们提供Subcloning Efficiency、Library Efficiency和MAX Efficiency感受态细胞。它们的区别是什么？

有少数例外，但他们主要的区别在于所保证的转化效率：

Subcloning Efficiency细胞每转化1 µg pUC19或pUC18超螺旋质粒保证产生至少1.0 x 10E6个转化子。
Library Efficiency细胞每转化1 µg pUC19或pUC18 DNA保证产生至少1.0 x 10E8个转化子。
MAX Efficiency细胞每转化1 µg pUC19或pUC18 DNA保证产生至少1.0 x 10E9个转化子。

You offer competent cells in Subcloning Efficiency, Library Efficiency and MAX Efficiency. How do these differ?

There are a few exceptions, but in general the difference is in guaranteed transformation efficiency as follows:

Subcloning Efficiency cells are guaranteed to produce at least 1.0 x 10E6 transformants per µg of transformed pUC19 or pUC18 supercoiled plasmid
Library Efficiency cells are guaranteed to produce at least 1.0 x 10E8 transformants per µg pUC19 or pUC18 DNA
MAX Efficiency cells are guaranteed to produce at least 1.0 x 10E9 transformants per µg pUC19 or pUC18 DNA