FreeStyle™ 293 Expression Medium

Gibco FreeStyle 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required.

Features of FreeStyle 293 Expression Medium include:

• Complete and ready-to-use format containing GlutaMAX supplement
• Ability to support suspension growth and transfection of 293-F cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use with the FreeStyle 293 expression systems
• Scalability in spinner flasks and bioreactors

Complete and ready-to-use format containing GlutaMAX supplement

FreeStyle 293 Expression Medium is ready to use. It does not require the addition of serum, glutamine, or surfactants. It contains GlutaMAX supplement to minimize toxic ammonia build-up and improve cell viability and growth in an easy-to-use format.

Ability to support suspension growth and transfection of 293-F cells

Gibco 293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle 293 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into FreeStyle 293 Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in FreeStyle 293 Expression Medium can be transfected using lipid based transfection reagents, such as 293fectin reagent (Cat. No. 12347019) and Freestyle MAX reagent (16447500).

Animal origin–free, protein-free and chemically defined formulation

FreeStyle 293 Expression Medium is animal origin–free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle 293 Expression System and FreeStyle MAX Expression System for protein production

The FreeStyle 293 Expression System (Cat. No. K900001) is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein (see figure). It combines FreeStyle 293 Expression Medium with 293fectin reagent, a cationic-lipid formulation designed to transfect suspension FreeStyle 293-F cells at high efficiency. The FreeStyle MAX Expression System is a breakthrough technology for rapid and high-yield mammalian protein production. It combines FreeStyle media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293-F cells.

Scalability in spinner flasks and bioreactors

Protein production using FreeStyle 293 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement the medium with additional Pluronic F-68 to avoid sheer stress in the culture.

Universal bag packaging

Gibco universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization.

Product use

Customers using FreeStyle 293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system

FreeStyle 293 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.