Recombination-based DNA assembly and mutagenesis methods for metabolic engineering.
AuthorsLiang X, Peng L, Tsvetanova B, Li K, Yang JP, Ho T, Shirley J, Xu L, Potter J, Kudlicki W, Peterson T, Katzen F
JournalMethods Mol Biol
PubMed ID22144356
'In recent years there has been a growing interest in the precise and concerted assembly of multiple DNA fragments of diverse sizes, including chromosomes, and the fine tuning of gene expression levels and protein activity. Commercial DNA assembly solutions have not been conceived to support the cloning of very large ... More
Evolution of human-chimpanzee differences in malaria susceptibility: relationship to human genetic loss of N-glycolylneuraminic acid.
AuthorsMartin MJ, Rayner JC, Gagneux P, Barnwell JW, Varki A,
JournalProc Natl Acad Sci U S A
PubMed ID16126901
Chimpanzees are the closest evolutionary cousins of humans, sharing >99% identity in most protein sequences. Plasmodium falciparum is the major worldwide cause of malaria mortality. Plasmodium reichenowi, a morphologically identical and genetically very similar parasite, infects chimpanzees but not humans. Conversely, experimental P. falciparum infection causes brief moderate parasitization and ... More