A rapid and general assay for monitoring endogenous gene modification.
Authors:Guschin DY, Waite AJ, Katibah GE, Miller JC, Holmes MC, Rebar EJ
Journal:Methods Mol Biol
PubMed ID:20680839
The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ... More
Generation of adult human induced pluripotent stem cells using nonviral minicircle DNA vectors.
Human induced pluripotent stem cells (hiPSCs) derived from patient samples have tremendous potential for innovative approaches to disease pathology investigation and regenerative medicine therapies. However, most hiPSC derivation techniques use integrating viruses, which may leave residual transgene sequences as part of the host genome, thereby unpredictably altering cell phenotype in ... More