293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:
• Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells • Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required • Add 293fectin™ reagent complexes directly to cells in culture medium • No need to remove complexes or change or add medium following transfection
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell Type
Established Cell Lines
Sample Type
Plasmid DNA
Transfection Technique
Lipid-based Transfection
For Use With (Application)
Transfection
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
293fectin™
Product Type
Transfection Reagent
Quantity
1 mL
Serum Compatible
Yes
Contents & Storage
Each unit contains one vial of reagent. Store at +4°C. 293fectin™ is guaranteed stable for 6 months when stored properly.