Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
AuthorsTakahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S,
JournalCell
PubMed ID18035408
Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation ... More
Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway(R) TOPO vector system.
AuthorsXu R, Li QQ,
JournalPlant Methods
PubMed ID18211693
ABSTRACT: BACKGROUND: In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway(R) cloning technology (Invitrogen Co.) has developed a fast and reliable alternative cloning methodology which uses ... More