• 2 x 1.25 mL Platinum SuperFi PCR 预混液 (2X) • 1.25 mL SuperFi GC 增强剂 • 2 x 1.25 mL 无核酸酶水
储存在 -5 至 30°C 下。
常见问题解答 (FAQ)
Which nucleotide analogues can be used with Platinum SuperFi DNA Polymerase?
Platinum SuperFi DNA Polymerase cannot read dUTP-derivatives or dITP in DNA templates, so the use of these analogues is not recommended.Platinum SuperFi DNA Polymerase can incorporate 7-deaza-dGTP and radiolabeled dNTPs.
I am having difficulties amplifying long targets using Platinum SuperFi DNA Polymerase. What are your recommendations?
Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh and intact) and fresh primer solutions. Optimization steps to consider include decreasing denaturation and extension temperatures, lengthening extension times as recommended in the manual, and increasing template amounts.
Can Platinum SuperFi DNA Polymerase amplify AT-rich targets?
Yes. To improve amplification of AT-rich targets, we recommend reducing the extension temperature to 68 degrees C or add 5-15 mM Tetramethylammonium Chloride (TMAC).
Can Platinum SuperFi DNA Polymerase amplify GC-rich targets?
All Platinum SuperFi product formats are supplied with 5X SuperFi GC Enhancer which is optimized to improve amplification of GC-rich targets (recommended for use with targets containing >65% GC content)
Does SuperFi Green Buffer influence performance of Platinum SuperFi DNA Polymerase?
No. The colored buffer does not interfere with PCR performance and does not change enzyme features.