A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation.
AuthorsKotaki T, Xie X, Shi PY, Kameoka M
JournalSci Rep
PubMed ID33500537
'The development of specific antiviral compounds to SARS-CoV-2 is an urgent task. One of the obstacles for the antiviral development is the requirement of biocontainment because infectious SARS-CoV-2 must be handled in a biosafety level-3 laboratory. Replicon, a non-infectious self-replicative viral RNA, could be a safe and effective tool for ... More
Engineering SARS-CoV-2 using a reverse genetic system.
AuthorsXie X, Lokugamage KG, Zhang X, Vu MN, Muruato AE, Menachery VD, Shi PY
JournalNat Protoc
PubMed ID33514944
'Reverse genetic systems are a critical tool for studying viruses and identifying countermeasures. In response to the ongoing COVID-19 pandemic, we recently developed an infectious complementary DNA (cDNA) clone for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The reverse genetic system can be used to rapidly engineer viruses with desired ... More
The analysis of GSTA1 promoter genetic and functional diversity of human populations.
AuthorsMlakar V, Curtis PH, Armengol M, Ythier V, Dupanloup I, Hassine KB, Lesne L, Murr R, Mlakar SJ, Nava T, Ansari M
JournalSci Rep
PubMed ID33658540
'GSTA1 encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. GSTA1 has several functional SNPs within its promoter region that are responsible for a change in its expression by altering promoter ... More
Epigallocatechin-3-gallate Alleviates Vanadium-Induced Reduction of Antioxidant Capacity via Keap1-Nrf2-sMaf Pathway in the Liver, Kidney, and Ovary of Laying Hens.
AuthorsMa Y, Shi Y, Wu Q, Ma W
JournalBiol Trace Elem Res
PubMed ID33405082
'This study evaluated the effect of epigallocatechin-3-gallate (EGCG) alleviating the reduction of antioxidant capacity induced by dietary vanadium (V) in the liver, kidney, and ovary of laying hens. Furthermore, Kelch-like ECH-associated protein 1(Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2)-small Maf proteins (sMaf) pathway was explored to reveal the molecular mechanism. ... More
Human Ubiquitin-Specific Peptidase 18 Is Regulated by microRNAs
AuthorsRubino E, Cruciani M, Tchitchek N, Le Tortorec A, Rolland AD, Veli Ö, Vallet L, Gaggi G, Michel F, Dejucq-Rainsford N, Pellegrini S
JournalFront Genet
PubMed ID33633774
'Ubiquitin-specific peptidase 18 (USP18) acts as gatekeeper of type I interferon (IFN) responses by binding to the IFN receptor subunit IFNAR2 and preventing activation of the downstream JAK/STAT pathway. In any given cell type, the level of USP18 is a key determinant of the output of IFN-stimulated transcripts. How the ... More
Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil.
AuthorsNascimento VAD, Corado ALG, Nascimento FOD, Costa ÁKAD, Duarte DCG, Luz SLB, Gonçalves LMF, Jesus MS, Costa CFD, Delatorre E, Naveca FG
JournalMem Inst Oswaldo Cruz
PubMed ID32997001
'A new coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] is currently causing a life-threatening pandemic. In this study, we report the complete genome sequencing and genetic characterisation of a SARS-CoV-2 detected in Manaus, Amazonas, Brazil, and the protocol we designed to generate high-quality SARS-CoV-2 full genome data. The isolate ... More
Evaluation of a Yeast Two-Hybrid Library by High-Throughput Sequencing.
AuthorsYu Q, Hu Y, Su J, Li P, Zhang L, Fu X, Chen F, Song A
JournalJ Proteome Res
PubMed ID32442380
'Yeast two-hybridization (Y2H) is a classical method to study protein-protein interactions in organisms, and the top limitation of Y2H is the high ratio of false negatives and false positives. The most efficient way to reduce this error is to improve the quality of the library. The traditional library quality evaluation ... More
Improved detection of differentially represented DNA barcodes for high-throughput clonal phenomics.
AuthorsAkimov Y, Bulanova D, Timonen S, Wennerberg K, Aittokallio T
JournalMol Syst Biol
PubMed ID32187448
'Cellular DNA barcoding has become a popular approach to study heterogeneity of cell populations and to identify clones with differential response to cellular stimuli. However, there is a lack of reliable methods for statistical inference of differentially responding clones. Here, we used mixtures of DNA-barcoded cell pools to generate a ... More
Iodide Transporters in the Endometrium: A Potential Diagnostic Marker for Women with Recurrent Pregnancy Failures.
'The element iodine is an essential nutrient utilized by the thyroid glands, and deficiency of this element has been linked to reproductive failures. Iodide transporters are also present in reproductive tissues and cells of embryonic origin such as the endometrium and trophoblasts, respectively. The aim of this study is to ... More
4C3 Human Monoclonal Antibody: A Proof of Concept for Non-pathogenic Proteinase 3 Anti-neutrophil Cytoplasmic Antibodies in Granulomatosis With Polyangiitis.
AuthorsGranel J, Lemoine R, Morello E, Gallais Y, Mariot J, Drapeau M, Musnier A, Poupon A, Pugnière M, Seren S, Nouar D, Gouilleux-Gruart V, Watier H, Korkmaz B, Hoarau C
JournalFront Immunol
PubMed ID33101296
Granulomatosis with polyangiitis (GPA) is a severe autoimmune vasculitis associated with the presence of anti-neutrophil cytoplasmic antibodies (ANCA) mainly targeting proteinase 3 (PR3), a neutrophilic serine proteinase. PR3-ANCA binding to membrane-bound PR3 on neutrophils induce their auto-immune activation responsible for vascular lesions. However, the correlation between PR3-ANCA level and disease ... More
An Infectious cDNA Clone of SARS-CoV-2.
AuthorsXie X, Muruato A, Lokugamage KG, Narayanan K, Zhang X, Zou J, Liu J, Schindewolf C, Bopp NE, Aguilar PV, Plante KS, Weaver SC, Makino S, LeDuc JW, Menachery VD, Shi PY
JournalCell Host Microbe
PubMed ID32289263
The ongoing pandemic of COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), underscores the urgency to develop experimental systems for studying this virus and identifying countermeasures. We report a reverse genetic system for SARS-CoV-2. Seven complimentary DNA (cDNA) fragments spanning the SARS-CoV-2 genome were assembled into a full-genome ... More
Genetic Characteristics of HIV-1 CRF12_BF First Identified in Guangdong Province, China.
AuthorsLan Y, He X, Xin R, Li L, Huang X, Deng X, Tang X, Jia W, Cai W, Hu F
JournalAIDS Res Hum Retroviruses
PubMed ID32873064
Multiple HIV-1 genotypes were found circulating in Guangdong Province, China, as this province is located in South China and has a high frequency of international trade. In this study, we report the near full-length genome (NFLG) of CRF12_BF that was identified from a male patient in Guangzhou city, Guangdong Province; ... More
New insight into microbial degradation of mycotoxins during anaerobic digestion.
AuthorsFerrara M, Haidukowski M, D'Imperio M, Parente A, De Angelis E, Monaci L, Logrieco AF, Mulè G
JournalWaste Manag
PubMed ID33070091
Anaerobic digestion represents an interesting approach to produce biogas from organic waste materials contaminated by mycotoxins. In this study a shotgun metagenomic analysis of lab-scale bioreactors fed with mycotoxin-contaminated silage has been carried out to characterize the evolution of microbial community under the operating conditions and the key enzymatic activities ... More
SARS-CoV-2 genomic surveillance in Rondônia, Brazilian Western Amazon.
AuthorsBotelho-Souza LF, Nogueira-Lima FS, Roca TP, Naveca FG, de Oliveria Dos Santos A, Maia ACS, da Silva CC, de Melo Mendonça ALF, Lugtenburg CAB, Azzi CFG, Fontes JLF, Cavalcante S, de Cássia Pontello Rampazzo R, Santos CHN, Di Sabatino Guimarães AP, Máximo FR, Villalobos-Salcedo JM, Vieira DS
JournalSci Rep
PubMed ID33580111
SARS-CoV-2 has spread rapidly around the world, with Brazil currently considered an epicenter of the pandemic. The Northern region has the second highest incidence coefficient, as well as the third highest mortality rate in the country. This study aimed to investigate information about the evolutionary history of epidemic spread and genetic aspects ... More