Evaluation of a Yeast Two-Hybrid Library by High-Throughput Sequencing.
AuthorsYu Q, Hu Y, Su J, Li P, Zhang L, Fu X, Chen F, Song A
JournalJ Proteome Res
PubMed ID32442380
'Yeast two-hybridization (Y2H) is a classical method to study protein-protein interactions in organisms, and the top limitation of Y2H is the high ratio of false negatives and false positives. The most efficient way to reduce this error is to improve the quality of the library. The traditional library quality evaluation ... More