BACKGROUND: The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However, the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA ... More
An in vivo model of human small intestine using pluripotent stem cells.
Differentiation of human pluripotent stem cells (hPSCs) into organ-specific subtypes offers an exciting avenue for the study of embryonic development and disease processes, for pharmacologic studies and as a potential resource for therapeutic transplant(1,2). To date, limited in vivo models exist for human intestine, all of which are dependent upon ... More
Serum-free media for the growth of primary bovine myoblasts.
Authors:Kolkmann AM,Post MJ,Rutjens MAM,van Essen ALM,Moutsatsou P
Journal:Cytotechnology
PubMed ID:31884572
The demand for meat is expected to exceed production capacity by livestock in the coming decennia. Therefore, cultured beef might be a viable alternative to traditional livestock-derived beef. One of the problems however is the sustainability of cultured beef through the use of fetal bovine serum. We aimed to identify ... More
miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin.
Authors:Zhuang J,Fan J,Zhu L,Zhao L,Huang Y,Pan X,Guo T
Journal:Journal of Cancer
PubMed ID:37497401
Background: Non-small cell lung cancer (NSCLC) is a common malignant tumor, and it is characterized by high mortality. MicroRNA-452-5p (miR-452-5p) and Moesin (MSN) have been proved to be related with regulation of tumors. If miR-452-5p could regulate NSCLC through targeting MSN remain unclear. Methods: TargetScan data and GEPIA databases were ... More
Understanding factors affecting human chondrocyte culturing: an experimental study.
Authors:Kilic P,Gurcan C,Gurman G,Yilmazer A
Journal:Cell and tissue banking
PubMed ID:32671509
Over the years, surgical strategies have been developed in hope of full regeneration of the injured cartilage. In our study, we aimed to develop an optimized chondrocyte culture isolation technique as an active ingredient of a standardized autologous chondrocte implantation product, which is able to maintain the phenotype along with ... More