Platinum™ PCR SuperMix High Fidelity, 5,000 reactions - FAQs

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5 个常见问题解答

Platinum 技术和 AccuPrime 技术有何差异？

使用Platinum 技术时，在94°C的变性步骤之前，抗DNA聚合酶的抗体结合在酶上。在变性步骤中，抗体将随之降解，聚合酶活化，引物开始介导延伸。AccuPrime Taq将PlatinumTaq (Taq + Platinum抗体) 与我们专利的热稳定 AccuPrime 辅助蛋白相结合。10x反应缓冲液中包含了该辅助蛋白，以提高每轮PCR反应中引物和模板结合的特异性。

使用Platinum Taq 高保真聚合酶时，我可以用MgCl2代替MgSO4吗？

我们强烈建议您使用MgSO4。尽管MgCl2在某些情况下也有效，但MgSO4通常可以获得更加稳健、可重复的产物——因为硫酸根是最适于Platinum Taq高保真聚合酶的阴离子。

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Can I use MgCl2 instead of MgSO4 with Platinum Taq High Fidelity enzyme?

We strongly suggest using MgSO4. While MgCl2 may work in some cases, MgSO4 usually produces more robust and reproducible products, as sulfate is the best anion found for the Platinum Taq High Fidelity enzyme.

If I do PCR using the Platinum PCR SuperMix High Fidelity, can I use DNA-RNA chimeric oligos such as 5'-AGGCTTggau (lower case letters for RNA bases)?

PCR cannot be performed with an RNA oligo. RNA is not heat stable so the RNA part of the oligo will be degraded. In the presence of Mg++ (PCR buffer contains usually 1-2 mM Mg++) the rate of degradation is much faster.