Improved full-length cDNA production based on RNA tagging by T4 DNA ligase.
AuthorsClepet C, Le Clainche I, Caboche M,
JournalNucleic Acids Res
PubMed ID14704363
Second-strand cDNA priming is a central problem for full-length characterization of transcripts. A new strategy using bacteriophage T4 DNA ligase and partially degenerate adapters is proposed for grafting a sequence tag to the end of polyribonucleotides. Based on this RNA tagging system and previously described protocols, a new method for ... More