High yield of biologically active recombinant human amelogenin using the baculovirus expression system.
AuthorsTaylor AL, Haze-Filderman A, Blumenfeld A, Shay B, Dafni L, Rosenfeld E, Leiser Y, Fermon E, Gruenbaum-Cohen Y, Deutsch D,
JournalProtein Expr Purif
PubMed ID16055347
The amelogenins are secreted by the ameloblast cells of developing teeth; they constitute about 90% of the enamel matrix proteins and play an important role in enamel biomineralization. Recent evidence suggests that amelogenin may also be involved in the regeneration of the periodontal tissues and that different isoforms may have ... More
A beta-1,3-N-acetylglucosaminyltransferase with poly-N- acetyllactosamine synthase activity is structurally related to beta-1,3- galactosyltransferases.
AuthorsZhou D, Dinter A, Gutierrez Gallego R, Kamerling JP, Vliegenthart JF, Berger EG, Hennet T
JournalProc Natl Acad Sci U S A
PubMed ID9892646
Human and mouse cDNAs encoding a new beta-1, 3-N- acetylglucosaminyltransferase (beta3GnT) have been isolated from fetal and newborn brain libraries. The human and mouse cDNAs included ORFs coding for predicted type II transmembrane polypeptides of 329 and 325 aa, respectively. The human and mouse beta3GnT homologues shared 90% similarity. The ... More