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View additional product information for TrypLE™ Select Enzyme (1X), no phenol red - FAQs (12563029, 12563011, 12563030, 12563031)
25 product FAQs found
缩短胰酶消化的时间，或使用更少量的胰酶进行处理，也可换用Gibco TrypLE Select或Gibco TrypLE Express。使用2-5 mL细胞生长培养基稀释胰酶处理体系后，将细胞悬液移至15 mL离心管中。以100x g离心5-10分钟。弃去上清液并使用2-5 mL新鲜培养基重悬细胞沉淀。确定细胞数量并按照常规方案将细胞接种入培养瓶，细胞应会正常贴壁。
是的，TrypLE产品含有重组蛋白酶，这是一款类似胰酶的非动物来源的重组酶，可用于解离贴壁细胞。经验证TrypLE能够有效解离无血清和含血清两种培养体系内的细胞。TrypLE Select的目录编号为12563-011（100 mL）和12563-029（500 mL）。TrypLE Express的部分目录编号包括不含酚红的12604-013（100 mL）与含酚红的12605-010（100 mL）。这两种配方均提供其他多种规格的产品。所有这些产品均不含动物源性成份。
这两款产品均含有重组蛋白酶，这是一款类似胰酶的非动物源性产品，可用于解离贴壁细胞。这款产品是利用微生物生产的重组酶，可替代猪源的胰酶产品。
Express与Select产品在生产与测试方法，以及提供的文件方面都有差别。TrypLE Select主要面向工业市场，通过了内毒素和酶活性的出厂测试。同时该产品也具有药物主文件记录。这款产品使用AOF专用设备进行生产，指定用途为研究级应用及进一步的细胞培养生产。
TrypLEExpress未使用专门设备进行生产，用途仅为科学研究。不适用于诊断操作。
如需阅读更多关于TrypLE产品的信息以及使用该试剂所需的细胞解离时间，请点击here(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).
TrypLE试剂不含动物源性与人源成份，纯度极高且对细胞的作用十分温和。该产品无需使用胰酶抑制剂对其进行终止。在含血清和不含血清的培养条件下，TrypLE试剂都是解离各类贴壁培养的哺乳动物细胞系的理想之选，能够直接做为胰酶替代品而应用于您当前的实验方案。
TrypLE试剂在常温下十分稳定，可随时按需取用。TrypLE细胞解离试剂能够在室温下稳定保存24个月，储存与操作都更为容易方便。请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。
是的，请访问此页面(https://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf)浏览我们的无酚红培养基页面。
可用的酶包括：Gibco TrypLE Express，Gibco TrypLE Select或Gibco StemPro Accutase 细胞解离试剂（Cell Dissociation Reagent）。
Gibco TrypLE Select酶（Gibco TrypLE Select Enzyme，货号 12563011）与Gibco StemPro Accutase细胞解离试剂（货号 A1110501）已测试过均能兼容Gibco Essential 8 Flex培养基。
Yes, TrypLE Select Enzyme can detach cells from glass surfaces.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The shelf life of TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011) is 24 months from the date of the manufacturer when stored at 15-30 degrees C, or 2-8 degrees C, and protected from light.
It can be stored at -5 to -20 degrees C for at least 22 months without affecting its performance.
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We do not have specific data on the light sensitivity of TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011). However, the protease generated by the confidential organism has been observed to be less effective when exposed to constant light. Therefore, we recommend storing the product away from light.
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We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.
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The following statement is regarding the customer’s responsibility to ensure the elimination of a reagent, such as the TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563011, 12563029), from their drug product and determination of drug product safety:
"Products are not manufactured by Thermo Fisher under a pharmaceutical quality management system to excipient requirements and are not intended to be used as excipients. Customers are responsible for properly qualifying any products it intends to use in or as excipients – this may include steps such as washing, filtration, and sterilization of these products."
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We recommend using TrypLE Express Enzyme or TrypLE Select Enzyme for passaging mesenchymal stem cells (MSCs). The usage of trypsin will result in lower cell viability upon passage. TrypLE Express and TrypLE Select are defined, animal origin-free, and stable at both room and refrigerated temperatures.
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TrypLE Select Enzyme has a shelf life of 24 months from the date of manufacture, when stored as recommended (15-30 degrees C).
There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.
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Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:
After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.
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Trypsinize this type of cells for a shorter duration or use less trypsin or Gibco TrypLE Select or Gibco TrypLE Express Trypsin Replacement. After trypsinization dilute in 2 to 5 mL of cell culture growth medium transfer the cell suspension to 15 mL centrifuge tube. Centrifuge for 5-10 minutes at 100 x g. Discard the supernatant and suspend cell pellet in 2-5 mL fresh medium. Determine the count and seed the flask according to normal protocol and cell should adhere to the surface.
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Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.
The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.
TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.
TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.
CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.
To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.
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Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).
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Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.
Find additional tips, troubleshooting help, and resources within our Extracellular Matrices (ECM) Support Center.
Gibco TrypLE Select Enzyme (Cat. No. 12563011) and Gibco StemPro Accutase Cell Dissociation Reagent (Cat. No A1110501) have been tested with Essential 8 Flex Medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.