Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.
AuthorsCheng Z, Ito S, Nishio N, Xiao H, Zhang R, Suzuki H, Okawa Y, Murohara T, Isobe K
JournalJ Mol Cell Biol
PubMed ID21228011
'If induced pluripotent stem (iPS) cells are to be used to treat damaged tissues or repair organs in elderly patients, it will be necessary to establish iPS cells from their tissues. To determine the feasibility of using this technology with elderly patients, we asked if it was indeed possible to ... More
A high-throughput automated platform for the development of manufacturing cell lines for protein therapeutics.
AuthorsShi S, Condon RG, Deng L, Saunders J, Hung F, Tsao YS, Liu Z
JournalJ Vis Exp
PubMed ID21968840
'The fast-growing biopharmaceutical industry demands speedy development of highly efficient and reliable production systems to meet the increasing requirement for drug supplies. The generation of production cell lines has traditionally involved manual operations that are labor-intensive, low-throughput and vulnerable to human errors. We report here an integrated high-throughput and automated ... More
Specific inhibition of ICAM-1 expression mediated by gene targeting with Triplex-forming oligonucleotides.
AuthorsBesch R, Giovannangeli C, Kammerbauer C, Degitz K
JournalJ Biol Chem
PubMed ID12080053
Selected sequences in the DNA double helix can be specifically recognized by oligonucleotides via hydrogen bonding interactions. The resulting triple helix can modulate DNA metabolism and especially interfere with transcription in a gene-specific manner. To explore the potential of triplex-forming oligonucleotides (TFOs) as gene repressors, a TFO was designed to ... More
Expression of human thyrotropin in cell lines with different glycosylation patterns combined with mutagenesis of specific glycosylation sites. Characterization of a novel role for the oligosaccharides in the in vitro and in vivo bioactivity.
Authors Grossmann M; Szkudlinski M W; Tropea J E; Bishop L A; Thotakura N R; Schofield P R; Weintraub B D;
JournalJ Biol Chem
PubMed ID7493973
We used a novel approach to study the role of the Asn-linked oligosaccharides for human thyrotropin (hTSH) activity. Mutagenesis of Asn (N) within individual glycosylation recognition sequences to Gln (Q) was combined with expression of wild type and mutant hTSH in cell lines with different glycosylation patterns. The in vitro ... More