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锚定 Oligo(dT)<sub>20</sub> 引物
Invitrogen™

锚定 Oligo(dT)20 引物

包括一串 20 个脱氧胸苷酸残基、随后的 dV（dG、dA 或 dC）以及之后的 dN（dA、dT、dG 或 dC）的引物混合物
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货号数量
1257701125 μL
货号 12577011
价格（CNY)
2,305.00
飞享价
Ends: 31-Dec-2026
3,137.00
共减 832.00 (27%)
Each
数量:
25 μL
价格（CNY)
2,305.00
飞享价
Ends: 31-Dec-2026
3,137.00
共减 832.00 (27%)
Each

Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (dG, dA, or dC) and then by dN (dA, dT, dG, or dC). Due to the variable end-sequence, the primer is anchored to the 5'-end of the poly(A) tail of mRNA and prevents priming within the poly(A) tail.

Anchored Oligo(dT)20 Primer is recommended for use in cDNA labeling protocols and in RT-PCR. It is commonly used for first-strand cDNA systhesis.

Quality control: This product is qualified in a first-strand cDNA synthesis reaction

由于可变端序列，引物被锚定至 mRNA 多聚腺苷酸尾的 5' 端，并阻止多聚腺苷酸尾内的引发。锚定 Oligo(dT)20 引物建议用于 cDNA 标记方案和 RT-PCR。

  • 质量控制：本产品在第一链 cDNA 合成反应中合格
  • 以 2.5 μg/μL DEPC 处理水溶液的形式提供

PCR & 实时荧光定量 PCR、逆转录

仅供科研使用。不可用于诊断程序。
规格
5' 引物修改磷酸盐
适用于（应用）第一链 cDNA 合成
引物长度22-mer
引物序列5'd PO4 [(T)20VN]3'
产品类型引物
纯化方法凝胶纯化，脱盐
数量25 μL
运输条件干冰
最大浓度2.5 μg/μL
引物Oligo dT
Unit SizeEach
内容与储存
锚定 寡核苷酸(dT)20 引物以 2.5 µg/µL DEPC 处理水溶液的形式提供。储存在 -20°C 下。妥善储存时，可保证稳定储存 6 个月。

常见问题解答 (FAQ)

How does the Anchored Oligo(dT)20 Primer differ from standard oligo(dT) primers?

Anchored Oligo(dT) primers have 2 random bases at the 3' end of the stretch of Ts. The first random base is either an A, G, or C, while the second can be any of the 4 standard nucleotides, i.e., A, T, G or C. The use of anchored oligo(dT) primers results in increased cDNA synthesis yields.

For first-strand cDNA synthesis, is it better to use oligo(dT), random hexamers, gene-specific primer (GSP), or combination of these primers?

The choice of primer depends on your experimental goals. Oligo(dT) is recommended when using total RNA for cDNA synthesis. It is the key to full-length cDNA synthesis. Random hexamers give a series of short first-strand products spanning the entire mRNA. Use of random hexamers may be helpful if the PCR fragment is at the 5´ end of a large mRNA. To ensure full-length cDNA synthesis of large transcripts, oligo(dT) can be added along with random hexamers during first-strand synthesis. Gene-specific primers (GSP) for cDNA synthesis may also be used and are required in a few applications such as 5´ RACE and qRT-PCR. For GC-rich templates or templates rich in secondary structure, a GSP may not work as well as priming with oligo dT for first-strand synthesis. If an RT-PCR is problematic, trying different options of oligo dT, random primers and/or GSP for priming first-strand synthesis may resolve the issue. Oligo(dT)20 primer (Cat. No. 18418-020) is recommended for use with SuperScript III Reverse Transcriptase (Cat. no. 18080-044), ThermoScript Reverse Transcriptase (Cat. No. 12236-014), Thermo-X Reverse Trascriptase (Cat. No. 11150-025), and Cloned AMV Reverse Transcriptase (Cat. No. 12328-019).

Reference:
Frohman,M.A., Dush,M.K., Martin, G.R. (1988) Proc. Nat. Acad. Sci USA 85, 8998