SUMO 蛋白酶
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SUMO 蛋白酶

SUMO 蛋白酶(也称为 Ulp)是酿酒酵母中 ULP1(Ubl 特异性蛋白酶 1)的重组片段了解更多信息
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货号数量
12588018250 units
货号 12588018
价格(CNY)
9,574.00
Each
添加至购物车
数量:
250 units
价格(CNY)
9,574.00
Each
添加至购物车
SUMO 蛋白酶(也称为 Ulp)是酿酒酵母中 ULP1(Ubl 特异性蛋白酶 1)的重组片段。其对 SUMO 蛋白融合具有高度特异性,识别 SUMO 的三级结构而不是氨基酸序列。SUMO 蛋白酶特点:

•高度活性切割
•无非特异性蛋白水解(高度特异性剪切*)
•在 2°C 至 37°C 下具有活性
• 六个组氨酸序列,有助于将其从酶切蛋白样品中去除

应用:从重组蛋白中去除融合标签
单位定义:一个 SUMO 蛋白酶单位定义为在30°C 下一小时内切割 2μg 底物蛋白的 85% 所需的酶量
质量控制:SUMO 蛋白酶的单条带纯度大于 85%,没有非特异性蛋白酶污染。其经过功能检测,不存在任何非特异性蛋白酶活性。
5 种酶
仅供科研使用。不可用于诊断程序。
规格
宿主种属酿酒酵母
数量250 units
运输条件干冰
Unit SizeEach
内容与储存
SUMO 蛋白酶与 10X 高盐和 10X 无盐稀释缓冲液一起提供。储存在 -80°C 下。如果正确储存,可保证稳定保存6个月。

常见问题解答 (FAQ)

Are NP40 and DTT required for SUMO Protease activity? Will they interfere with downstream applications?

Yes, they are both required, although it might be possible to substitute the NP40 with another detergent via dialysis if you have concern about interference with mass spectrometry. DTT is an absolute requirement.

What enzyme should I choose if I do not want any extra amino acids left on my protein of interest after cleavage?

We would recommend using our SUMO system.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does SUMO protease work?

SUMO protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence. The SUMO protease itself has a His tag for easy removal from the protein mix after cleavage.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What products do you offer for enzymatic cleavage of fusion tags from recombinant proteins?

We offer the following products:

-AcTEV Protease (Cat. Nos. 12575015, 12575023)
-EKMax Enterokinase (Cat. Nos. E18001, E18002)
-SUMO Protease (Cat. No. 12588018)


Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.